CHARACTERIZATION OF A TEMPERATE BACTERIOPHAGE OF LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS AND ITS INTERACTIONS WITH THE HOST-CELL CHROMOSOME

被引：18

作者：

LAHBIBMANSAIS, Y ^{[1
]}

BOIZET, B ^{[1
]}

DUPONT, L ^{[1
]}

MATA, M ^{[1
]}

RITZENTHALER, P ^{[1
]}

机构：

[1] CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,F-31062 TOULOUSE,FRANCE

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷

关键词：

D O I：

10.1099/00221287-138-6-1139

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The temperate bacteriophage mv4 is representative of a widespread phage genetic group of Lactobacillus delbrueckii subsp. lactis or bulgaricus. The genome of this phage is circularly permuted and terminally redundant, as shown by mature mv4 homoduplexes, Southern hybridization and restriction enzyme analysis. A circular map of the mv4 genome was established, with unique sequences totalling 36 kb. The genomic location of the pac site (packaging site of mv4 DNA into phage heads) and the att site (phage integration site into the host cell chromosome) was determined. The genes coding for the two main structural phage proteins and for a phage-associated lysin were also mapped. Phage mv4 is capable of transducing a limited set of pieces of bacterial DNA and several specific chromosomal attachment sites of phage mv4 were identified. Bacteria lysogenic for phage mv4 were shown to be immune to infection by L. delbrueckii virulent phages related to mv4.

引用

页码：1139 / 1146

页数：8

共 41 条

[11] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[12]

HOTTINGER H, 1987, GENE, V60, P149

[13] ECORI ANALYSIS OF BACTERIOPHAGE-P22 DNA PACKAGING [J].

JACKSON, EN ;

JACKSON, DA ;

DEANS, RJ .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 118 (03) :365-388

[14]

Johnson D. A., 1984, Gene Analysis Techniques, V1, P3, DOI 10.1016/0735-0651(84)90049-9

[15] TERMINAL REDUNDANCY AND CIRCULAR PERMUTATION OF MYCOPLASMA VIRUS L3 DNA [J].

JUST, W ;

KLOTZ, G .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2157-2162

[16] MOLECULAR TAXONOMY OF LACTOBACILLUS PHAGES [J].

LAHBIBMANSAIS, Y ;

MATA, M ;

RITZENTHALER, P .

BIOCHIMIE, 1988, 70 (03) :429-435

[17] CIRCULAR PERMUTATION OF THE GENOME OF A TEMPERATE BACTERIOPHAGE FROM STREPTOCOCCUS-CREMORIS BK5 [J].

LAKSHMIDEVI, G ;

DAVIDSON, BE ;

HILLIER, AJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (04) :1039-1045

[18]

LANGELLA P, 1987, FEMS MICROBIOLOGY LE, V60, P149

[19] GENOME COMPARISON OF LACTOCOCCUS STRAINS BY PULSED-FIELD GEL-ELECTROPHORESIS [J].

LEBOURGEOIS, P ;

MATA, M ;

RITZENTHALER, P .

FEMS MICROBIOLOGY LETTERS, 1989, 59 (1-2) :65-69

[20]

MASTERS M, 1985, GENETICS BACTERIA, P197

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