PROTON NMR-STUDIES OF MANGANESE ION-BINDING TO TRANSFER-RNA-DERIVED ACCEPTOR ARM DUPLEXES

被引：42

作者：

OTT, G ^{[1
]}

ARNOLD, L ^{[1
]}

LIMMER, S ^{[1
]}

机构：

[1] UNIV BAYREUTH, BIOCHEM LAB, D-95440 BAYREUTH, GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 25期

关键词：

D O I：

10.1093/nar/21.25.5859

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several RNA duplexes corresponding to the acceptor arms of different tRNAs have been analyzed with respect to their divalent metal ion binding capability by means of proton NMR spectroscopy using paramagnetic Mn2+ ions as probes. In particular, the role of GU wobble base pairs has been analyzed with reference to their potential for creating metal ion binding sites. It is shown that both the structural modifications induced by GU pairs in the A-RNA geometry and the sequence context seem to affect the metal ion binding capabilities.

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页码：5859 / 5864

页数：6

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