OLIGONUCLEOTIDIC COMPOUNDS .75. DIMETHYLAMINOMETHYLENE PROTECTED PURINE H-PHOSPHONATES IN THE SYNTHESIS OF BIOLOGICALLY-ACTIVE RNA (24-MER)

被引：15

作者：

ARNOLD, L ^{[1
]}

SMRT, J ^{[1
]}

ZAJICEK, J ^{[1
]}

OTT, G ^{[1
]}

SCHIESSWOHL, M ^{[1
]}

SPRINZL, M ^{[1
]}

机构：

[1] UNIV BAYREUTH, BIOCHEM LAB, W-8580 BAYREUTH, GERMANY

来源：

COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS | 1991年 / 56卷 / 09期

关键词：

D O I：

10.1135/cccc19911948

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Preparation of 5'-O-(4,4'-dimethoxytrityl)-2'-O-tert-butyldimethylsilyl (tBDMSi) derivatives of N2-dimethylaminomethyleneguanosine (IIIa) and N6-dimethylaminomethyleneadenosine (IVa) and their 3'-H-phosphonates (IIIb, IVb) is described. The compounds IIIb and IVb together with corresponding derivatives of uridine (Vb) and N4-benzoylcytidine (VIb) were used as synthones in machine assisted synthesis of microhelix(Ala) (5'-GGGGCUAUAGCUCUAGCU. CCACCA-3') (X). The compound X was aminoacylated by means of alanyl-t-RNA synthetase (E. coli).

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页码：1948 / 1956

页数：9

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