NEURONS DERIVED FROM EMBRYONAL CARCINOMA (P19) CELLS EXPRESS MULTIPLE GABA(A) RECEPTOR SUBUNITS AND FULLY FUNCTIONAL GABA(A) RECEPTORS

被引：22

作者：

REYNOLDS, JN

RYAN, PJ

PRASAD, A

PATERNO, GD

机构：

[1] Faculty of Medicine, Health Sciences Centre, Memorial University of Newfoundland, St. John's, Nfld. A1B 3V6

来源：

NEUROSCIENCE LETTERS | 1994年 / 165卷 / 1-2期

关键词：

GABA(A) RECEPTOR; MESSENGER RNA; BENZODIAZEPINE; P19 CELL LINE; VOLTAGE-CLAMP; CHLORIDE CURRENT;

D O I：

10.1016/0304-3940(94)90726-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The embryonal carcinoma (EC) cell line P19 can be induced to differentiate into neurons and glia by treatment with retinoic acid. Neuronal cells derived from P19 stem cells were found to express messenger RNAs for alpha, beta, and gamma(2) subunits of the GABA(A) receptor-chloride channel complex. Whole-cell voltage-clamp recording in differentiated P19 cells revealed that these cells possess GABA receptor-activated chloride currents which are blocked by bicuculline and potentiated by flurazepam. P19 EC cells thus represent a stable neuronal cell line which expresses functional GABA(A) receptors with all of the characteristics of native GABA(A) receptors.

引用

页码：129 / 132

页数：4

共 23 条

[1] THE HUMAN NEUROBLASTOMA CELL-LINE, IMR-32 POSSESSES A GABA(A) RECEPTOR LACKING THE BENZODIAZEPINE MODULATORY SITE [J].

ANDERSON, SMP ;

DESOUZA, RJ ;

CROSS, AJ .

NEUROPHARMACOLOGY, 1993, 32 (05) :455-460

[2]

ANGELOTTI TP, 1993, J NEUROSCI, V13, P1429

[3]

ANGELOTTI TP, 1993, J NEUROSCI, V13, P1418

[4] EXPRESSION OF THE GENES-CODING FOR GLUTAMIC-ACID DECARBOXYLASE IN PLURIPOTENT CELL-LINES [J].

BAIN, G ;

RAMKUMAR, TP ;

CHENG, JM ;

GOTTLIEB, DI .

MOLECULAR BRAIN RESEARCH, 1993, 17 (1-2) :23-30

[5] DIFFERENTIAL LOCALIZATION OF 2 ALTERNATIVELY SPLICED GABA-A RECEPTOR GAMMA-2-SUBUNIT MESSENGER-RNAS IN THE CHICK BRAIN [J].

GLENCORSE, TA ;

BATESON, AN ;

DARLISON, MG .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1992, 4 (03) :271-277

[6]

HALES TG, 1992, MOL PHARMACOL, V42, P197

[7] RETINOIC ACID INDUCES EMBRYONAL CARCINOMA-CELLS TO DIFFERENTIATE INTO NEURONS AND GLIAL-CELLS [J].

JONESVILLENEUVE, EMV ;

MCBURNEY, MW ;

ROGERS, KA ;

KALNINS, VI .

JOURNAL OF CELL BIOLOGY, 1982, 94 (02) :253-262

[8] CLONING AND DEVELOPMENTAL EXPRESSION OF THE MURINE NEUROFILAMENT GENE FAMILY [J].

JULIEN, JP ;

MEYER, D ;

FLAVELL, D ;

HURST, J ;

GROSVELD, F .

MOLECULAR BRAIN RESEARCH, 1986, 1 (03) :243-250

[9] EXPRESSION OF GABA-A RECEPTOR POLYPEPTIDES IN CLONAL RAT-CELL LINES [J].

KASCKOW, JW ;

TILLAKARATNE, NJK ;

KIM, H ;

STRECKER, GJ ;

TOBIN, AJ ;

OLSEN, RW .

BRAIN RESEARCH, 1992, 581 (01) :143-147

[10]

KAWASAKI ES, 1989, PCR TECHNOLOGY PRINC, P89

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