TOWARDS A DNA VACCINE AGAINST TUBERCULOSIS

被引：87

作者：

LOWRIE, DB ^{[1
]}

TASCON, RE ^{[1
]}

COLSTON, MJ ^{[1
]}

SILVA, CL ^{[1
]}

机构：

[1] UNIV SAO PAULO,SCH MED,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-05508 SAO PAULO,BRAZIL

来源：

VACCINE | 1994年 / 12卷 / 16期

关键词：

TUBERCULOSIS; HEAT-SHOCK PROTEIN 65; CELL-MEDIATED PROTECTION;

D O I：

10.1016/0264-410X(94)90080-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Expression of the gene for a single mycobacterial antigen (Mycobacterium leprae hsp65) in adult Balb/c mice resulted in substantial cell-mediated protection against challenge with M. tuberculosis. CD4 and CD8 T cells cloned from spleens of such immunized mice passively transferred protection to non-immunized mice, and CD8 cells selectively lysed macrophages infected with M. tuberculosis. Three modes of expressing the gene have been tested: (1) expression from a retroviral vector (pZIPNeoSV) in implanted J774 tumour cells, (2) expression from the same vector via bone marrow cells transfected in vitro and used to reconstitute irradiated mice, and (3) in a preliminary experiment, from CMV immediate-early and hydroxymethylglutaryl Co-A reductase promoters injected as plasmid DNA into muscle.

引用

页码：1537 / 1540

页数：4

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