MOLECULAR-CLONING AND CHARACTERIZATION OF PROLYL ENDOPEPTIDASE FROM HUMAN T-CELLS

被引:44
作者
SHIRASAWA, Y
OSAWA, T
HIRASHIMA, A
机构
[1] Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186
关键词
HUMAN T-CELL; MEMBRANE PROTEIN; PEPTIDASE; PROLYL ENDOPEPTIDASE;
D O I
10.1093/oxfordjournals.jbchem.a124402
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The prolyl endopeptidase (PEP) gene of human T cells was amplified by the PCR method and cloned in Escherichia coli. The complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. The nucleotide sequence of this clone revealed that T cell PEP DNA is 48, 50, and 91% homologous to those of Flavobacterium meningosepticum, Aeromonas hydrophila, and porcine brain PEP, respectively. This gene was fused to the lacZ sequence from E. coli and expressed as a fused protein in E. coli. This fused protein exhibited PEP activity, which was inhibited by Z-Pro-prolinal, a specific inhibitor of PEP. The fused protein was purified on a P-galactosidase specific affinity column. A polyclonal antibody was raised against the purified protein. Immunological characterization suggested that this protein is different from cytosol-soluble PEP.
引用
收藏
页码:724 / 729
页数:6
相关论文
共 20 条
[11]   POST-PROLINE ENDOPEPTIDASE IN HUMAN-PLACENTA [J].
MIZUTANI, S ;
SUMI, S ;
SUZUKI, O ;
NARITA, O ;
TOMODA, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 786 (1-2) :113-117
[12]   PORCINE MUSCLE PROLYL ENDOPEPTIDASE AND ITS ENDOGENOUS SUBSTRATES [J].
MORIYAMA, A ;
NAKANISHI, M ;
SASAKI, M .
JOURNAL OF BIOCHEMISTRY, 1988, 104 (01) :112-117
[13]   PORCINE LIVER SUCCINYLTRIALANINE PARA-NITROANILIDE HYDROLYTIC ENZYME - ITS PURIFICATION AND CHARACTERIZATION AS A POST-PROLINE CLEAVING ENZYME [J].
MORIYAMA, A ;
SASAKI, M .
JOURNAL OF BIOCHEMISTRY, 1983, 94 (05) :1387-1397
[14]   CDNA CLONING OF PORCINE BRAIN PROLYL ENDOPEPTIDASE AND IDENTIFICATION OF THE ACTIVE-SITE SERYL RESIDUE [J].
RENNEX, D ;
HEMMINGS, BA ;
HOFSTEENGE, J ;
STONE, SR .
BIOCHEMISTRY, 1991, 30 (08) :2195-2203
[15]  
SOEDA S, 1984, CHEM PHARM BULL, V32, P4061
[16]  
SUDO J, 1985, CHEM PHARM BULL, V33, P1694
[17]   A COMPARISON OF THE PROPERTIES AND ENZYMATIC-ACTIVITIES OF 3 ANGIOTENSIN PROCESSING ENZYMES - ANGIOTENSIN-CONVERTING ENZYME, PROLYL ENDOPEPTIDASE AND NEUTRAL ENDOPEPTIDASE 24.11 [J].
WELCHES, WR ;
BROSNIHAN, KB ;
FERRARIO, CM .
LIFE SCIENCES, 1993, 52 (18) :1461-1480
[18]   PROLYL ENDOPEPTIDASE FROM FLAVOBACTERIUM-MENINGOSEPTICUM - CLONING AND SEQUENCING OF THE ENZYME GENE [J].
YOSHIMOTO, T ;
KANATANI, A ;
SHIMODA, T ;
INAOKA, T ;
KOKUBO, T ;
TSURU, D .
JOURNAL OF BIOCHEMISTRY, 1991, 110 (06) :873-878
[19]   SPECIFIC INHIBITORS FOR PROLYL ENDOPEPTIDASE AND THEIR ANTI-AMNESIC EFFECT [J].
YOSHIMOTO, T ;
KADO, K ;
MATSUBARA, F ;
KORIYAMA, N ;
KANETO, H ;
TSURU, D .
JOURNAL OF PHARMACOBIO-DYNAMICS, 1987, 10 (12) :730-735
[20]   HUMAN-LUNG POSTPROLINE ENDOPEPTIDASE - PURIFICATION AND ACTION ON VASOACTIVE PEPTIDES [J].
ZOLFAGHARI, R ;
BAKER, CRF ;
CANIZARO, PC ;
FEOLA, M ;
AMIRGHOLAMI, A ;
BEHAL, FJ .
ENZYME, 1986, 36 (03) :165-178