INVITRO FERTILIZATION OF PIG OOCYTES AFTER DIFFERENT COINCUBATION INTERVALS

被引：28

作者：

COY, P ^{[1
]}

MARTINEZ, E ^{[1
]}

RUIZ, S ^{[1
]}

VAZQUEZ, JM ^{[1
]}

ROCA, J ^{[1
]}

MATAS, C ^{[1
]}

PELLICER, MT ^{[1
]}

机构：

[1] MURCIA UNIV,FAC VET,DEPT ANIM PATHOL,E-30100 MURCIA,SPAIN

来源：

THERIOGENOLOGY | 1993年 / 39卷 / 05期

关键词：

OOCYTE; INVITRO FERTILIZATION; COINCUBATION TIME; PIG;

D O I：

10.1016/0093-691X(93)90018-Z

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of gamete coincubation time on porcine in vitro fertilization for 4, 6 or 8 hours in Trial 1 or for 1, 2, 3 or 4 hours in Trial 2 were studied. The 876 oocytes were recovered from oviducts of prepubertal gilts. Ovulation was induced. Excessive spermatozoa attached to zona pellucida were removed by pippeting after coincubation (1 to 8 hours). Optimum results were obtained after 3 to 4 hours of coincubation, when 23 to 29% of the oocytes were fertilized by a single spermatozoa. Shorter intervals of coincubation were characterized by reduced percentages of fertilized oocytes and longer intervals of coincubation by increased rates of polyspermic fertilization. The employed sperm concentration was deliberately high (2 x 10(6) sperm/ml). The results show that a coincubation time of 4 hours may be optimal for pig in vitro fertilization. Under these assay conditions, modification of other factors such as sperm concentration, medium volume and physiological environment may increase the percentage of monospermic fertilization.

引用

页码：1201 / 1208

页数：8