IN-VIVO STUDY OF MIGM AND MIGD CROSS-LINKING ON MURINE B-CELLS

被引：14

作者：

DENIS, O ^{[1
]}

MACEDOSOARES, F ^{[1
]}

LATINNE, D ^{[1
]}

NISOL, F ^{[1
]}

BAZIN, H ^{[1
]}

机构：

[1] INST BUTANTAN,IMMUNOPATHOL LAB,SAO PAULO,SP,BRAZIL

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1994年 / 39卷 / 06期

关键词：

D O I：

10.1111/j.1365-3083.1994.tb03423.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we have analysed in mice the effects on the immune response of in vivo treatment with different rat monoclonal antibodies (MoAb) against IgM and IgD. Although the effects of IgD crosslinking have been studied already, no attempt has been made to characterize the effects of in vivo IgM crosslinking, probably because of the higher IgM serum levels compared to IgD. We have used a panel of nine monoclonal rat anti-mouse IgM and three anti-IgD antibodies and we have characterized their isotypes, avidities, immunoglobulin (Ig) cross-linking and internalization abilities. Our results show that injection of mice with some rat MoAb against IgM led to an important decrease of IgM serum level and internalization of membrane IgM (mIgM) on almost all B cells. Similarly, treatment with a high-avidity anti-IgD antibody induced disappearance of mIgD on B cells. Treatment with rat MoAb against IgM or IgD led to a synthesis of specific antibodies and there was a direct relationship between the Ig internalization abilities of rat MoAb and the induction of specific antibody production. Finally, treatment with a high-avidity rat MoAb against IgD induced a polyclonal IgE and IgG1 secretion. The significance of these results on mig receptor functions is discussed.

引用

页码：625 / 632

页数：8

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