AROMATIC RINGS OF TYROSINE RESIDUES AT ADENINE-NUCLEOTIDE BINDING-SITES OF THE BETA-SUBUNITS OF F1-ATPASE ARE NOT NECESSARY FOR ATPASE ACTIVITY

被引：11

作者：

ODAKA, M

KOBAYASHI, H

MUNEYUKI, E

YOSHIDA, M

机构：

[1] TOKYO INST TECHNOL,DEPT LIFE SCI,NAGATSUTA 4259,YOKOHAMA,KANAGAWA 227,JAPAN

[2] RIKKYO UNIV,DEPT CHEM,TOKYO 171,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91718-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using site-directed mutagenesis, Tyr-307, Tyr-341, or Tyr-364, supposedly located at the adenine nucleotide binding site(s) of the β subunits of F1-ATPase from the thermophilic bacterium PS3, was replaced with Phe or Cys. The α3β3 complexes reconstituted from the α subunits and individual mutant β subunits hydrolyzed ATP. Thus, neither the hydroxyl groups nor the aromatic rings in these positions are required for ATPase activity of F1-ATPase. © 1990.

引用

收藏

页码：372 / 378

页数：7

相关论文

共 29 条

[11] IDENTIFICATION OF AMINO-ACID-RESIDUES PHOTOLABELED WITH 2-AZIDO [ALPHA-P-32] ADENOSINE-DIPHOSPHATE IN THE BETA-SUBUNIT OF BEEF-HEART MITOCHONDRIAL F1-ATPASE [J].

GARIN, J ;

BOULAY, F ;

ISSARTEL, JP ;

LUNARDI, J ;

VIGNAIS, PV .

BIOCHEMISTRY, 1986, 25 (15) :4431-4437

[12] EVOLUTION OF THE VACUOLAR H+-ATPASE - IMPLICATIONS FOR THE ORIGIN OF EUKARYOTES [J].

GOGARTEN, JP ;

KIBAK, H ;

DITTRICH, P ;

TAIZ, L ;

BOWMAN, EJ ;

BOWMAN, BJ ;

MANOLSON, MF ;

POOLE, RJ ;

DATE, T ;

OSHIMA, T ;

KONISHI, J ;

DENDA, K ;

YOSHIDA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6661-6665

[13]

HOLLEMANS M, 1983, J BIOL CHEM, V258, P9307

[14] ALPHA-3-BETA-3 COMPLEX OF THERMOPHILIC ATP SYNTHASE - CATALYSIS WITHOUT THE GAMMA-SUBUNIT [J].

KAGAWA, Y ;

OHTA, S ;

OTAWARAHAMAMOTO, Y .

FEBS LETTERS, 1989, 249 (01) :67-69

[15] STABLE STRUCTURE OF THERMOPHILIC PROTON ATPASE BETA-SUBUNIT [J].

KAGAWA, Y ;

ISHIZUKA, M ;

SAISHU, T ;

NAKAO, S .

JOURNAL OF BIOCHEMISTRY, 1986, 100 (04) :923-934

[16]

KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367

[17]

MESSING J, 1983, METHOD ENZYMOL, V101, P20

[18] THE ALPHA-3-BETA-3 COMPLEX, THE CATALYTIC CORE OF F1-ATPASE [J].

MIWA, K ;

YOSHIDA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6484-6487

[19] SEQUENCE AND OVER-EXPRESSION OF SUBUNITS OF ADENOSINE-TRIPHOSPHATE SYNTHASE IN THERMOPHILIC BACTERIUM PS3 [J].

OHTA, S ;

YOHDA, M ;

ISHIZUKA, M ;

HIRATA, H ;

HAMAMOTO, T ;

OTAWARAHAMAMOTO, Y ;

MATSUDA, K ;

KAGAWA, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 933 (01) :141-155

[20] INVITRO MUTATED BETA-SUBUNITS FROM THE F1-ATPASE OF THE THERMOPHILIC BACTERIUM, PS3, CONTAINING GLUTAMINE IN PLACE OF GLUTAMIC-ACID IN POSITIONS 190 OR 201 ASSEMBLES WITH THE ALPHA-SUBUNITS AND GAMMA-SUBUNITS TO PRODUCE INACTIVE COMPLEXES [J].

OHTSUBO, M ;

YOSHIDA, M ;

OHTA, S ;

KAGAWA, Y ;

YOHDA, M ;

DATE, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (02) :705-710