A LIPID-CONJUGATED IMMUNOSTIMULATING COMPLEX SUBUNIT VACCINE AGAINST SALMONELLA INFECTION IN TURKEYS

被引:17
作者
CHARLES, SD
NAGARAJA, KV
SIVANANDAN, V
机构
[1] Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Paul
关键词
D O I
10.2307/1591675
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Immunostaining complexes (ISCOMs) are multimeric particles and have been used successfully for presentation of membrane proteins. In this study, outer-membrane proteins (OMPs) from Salmonella heidelberg were incorporated into lipid-conjugated ISCOM particles and evaluated for their use in a vaccine for turkeys against homologous and heterologous Salmonella challenge. Two types of lipid-conjugated ISCOMs were examined: ISCOM-phospholipid and ISCOM-sphingolipid preparations. The turkeys were challenged with one of the three Salmonella serotypes: S. heidelberg, S. reading, or S. enteritidis. The turkeys were monitored for clinical signs, shedding pattern post-challenge, and clearance of the challenge Salmonella from selected internal organs. Vaccines containing OMP with either lipid-conjugated ISCOM preparation produced significantly greater (P < 0.01) immune response than OMP alone. Cloacal swabs from turkeys given OMP along with ISCOM-phospholipid and challenged with a homologous serotype were completely negative for Salmonella. A certain degree of cross-protection against heterologous Salmonella was afforded by both OMP-ISCOM vaccines. The isolation rate of Salmonella from internal organs was significantly lower (P < 0.0001) in vaccinated turkeys than in unvaccinated controls.
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页码:477 / 484
页数:8
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