EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT

被引：11

作者：

KURSCHAT, P

GRAEVE, L

ERREN, A

GATSIOS, P

ROSEJOHN, S

ROEB, E

TSCHESCHE, H

KOJ, A

HEINRICH, PC

机构：

[1] RHEIN WESTFAL TH AACHEN,KLINIKUM,INST BIOCHEM,D-52057 AACHEN,GERMANY

[2] UNIV MAINZ,MED CLIN 1,PATHOPHYSIOL SECT,W-6500 MAINZ,GERMANY

[3] UNIV BIELEFELD,FAK CHEM,LEHRSTUHL BIOCHEM,W-4800 BIELEFELD,GERMANY

[4] JAGIELLONIAN UNIV,JAN ZURZYCKI INST MOLEC BIOL,KRAKOW,POLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 234卷 / 02期

关键词：

METALLOPROTEINASES; BACULOVIRUS; N-GLYCOSYLATION; COLLAGENASE; TISSUE INHIBITOR OF METALLOPROTEINASE-1;

D O I：

10.1111/j.1432-1033.1995.485_b.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine tissue inhibitor of metalloproteinases-1 (mTIMP-1) was expressed in baculovirus-infected insect cells (Sf9). The protein secreted into the culture medium was purified to homogeneity by means of heparin-Sepharose CL-6B and FPLC. The purified protein showed metalloproteinase-inhibitory activity in two independent assays: reverse zymography and inhibition of collagenase activity. Digestion of the recombinant TIMP-1 with peptide-N-glycanaseF revealed that both N-glycosylation sites are used. I-125-mTIMP-1 intraveneously injected into a male Sprague Dawley rat disappeared within 2 min from the circulation. 5 min after injection more than 50% of the I-125-mTIMP-1 were found in the liver and 20% in the kidneys. At later times, trichloroacetic-acid-soluble material accumulated in the intestinal tract.

引用

页码：485 / 491

页数：7

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