SUBTILISIN - AN ENZYME DESIGNED TO BE ENGINEERED

被引：256

作者：

WELLS, JA ^{[1
]}

ESTELL, DA ^{[1
]}

机构：

[1] GENENCOR INC,DEPT RES,SAN FRANCISCO,CA 94080

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1988年 / 13卷 / 08期

关键词：

D O I：

10.1016/0968-0004(88)90121-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：291 / 297

页数：7

共 33 条

[1] REFINED 1.2-A CRYSTAL-STRUCTURE OF THE COMPLEX FORMED BETWEEN SUBTILISIN CARLSBERG AND THE INHIBITOR EGLIN-C - MOLECULAR-STRUCTURE OF EGLIN AND ITS DETAILED INTERACTION WITH SUBTILISIN [J].

BODE, W ;

PAPAMOKOS, E ;

MUSIL, D ;

SEEMUELLER, U ;

FRITZ, H .

EMBO JOURNAL, 1986, 5 (04) :813-818

[2]

BOTT R, 1988, J BIOL CHEM, V263, P7895

[3]

BOTT R, 1987, ACS S SERIES, V334, P137

[4] SITE-DIRECTED MUTAGENESIS AND THE ROLE OF THE OXYANION HOLE IN SUBTILISIN [J].

BRYAN, P ;

PANTOLIANO, MW ;

QUILL, SG ;

HSIAO, HY ;

POULOS, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3743-3745

[5]

BRYAN P N, 1986, Proteins Structure Function and Genetics, V1, P326

[6] ENGINEERING ENZYME SPECIFICITY BY SUBSTRATE-ASSISTED CATALYSIS [J].

CARTER, P ;

WELLS, JA .

SCIENCE, 1987, 237 (4813) :394-399

[7] DISSECTING THE CATALYTIC TRIAD OF A SERINE PROTEASE [J].

CARTER, P ;

WELLS, JA .

NATURE, 1988, 332 (6164) :564-568

[8] IMPROVEMENT IN THE ALKALINE STABILITY OF SUBTILISIN USING AN EFFICIENT RANDOM MUTAGENESIS AND SCREENING-PROCEDURE [J].

CUNNINGHAM, BC ;

WELLS, JA .

PROTEIN ENGINEERING, 1987, 1 (04) :319-325

[9]

ESTELL DA, 1985, J BIOL CHEM, V260, P6518

[10] PROBING STERIC AND HYDROPHOBIC EFFECTS ON ENZYME-SUBSTRATE INTERACTIONS BY PROTEIN ENGINEERING [J].

ESTELL, DA ;

GRAYCAR, TP ;

MILLER, JV ;

POWERS, DB ;

BURNIER, JP ;

NG, PG ;

WELLS, JA .

SCIENCE, 1986, 233 (4764) :659-663

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