EXPERIMENTAL EVALUATION OF SORBENT BED HOMOGENEITY IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC COLUMNS

被引：7

作者：

MACKO, T ^{[1
]}

BEREK, D ^{[1
]}

机构：

[1] SLOVAK ACAD SCI, INST POLYMER, DUBRAVSKA CESTA 10, CS-84236 BRATISLAVA, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 592卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85079-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Base-line perturbations, eigenzones, are generated in a high-performance liquid chromatographic (HPLC) column by sudden pressure variations if particular binary eluents are used. The proposed interpretation of the shape of these eigenzones allows the semiquantitative evaluation of the packing density distribution along the column. Common HPLC equipment allows the necessary experimental data to be obtained, and their evaluation is simple and straightforward. However, the correction of eigenzones for spreading is necessary in order to draw more reliable, quantitative conclusions.

引用

页码：109 / 119

页数：11

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