DNA STRAND BREAKAGE BY HYDROXYPHENYL RADICALS GENERATED FROM MUTAGENIC DIAZOQUINONE COMPOUNDS

被引：42

作者：

KATO, T ^{[1
]}

KOJIMA, K ^{[1
]}

HIRAMOTO, K ^{[1
]}

KIKUGAWA, K ^{[1
]}

机构：

[1] TOKYO COLL PHARM,1432-1 HORINOUCHI,HACHIOJI,TOKYO 19203,JAPAN

来源：

MUTATION RESEARCH | 1992年 / 268卷 / 01期

关键词：

PARADIAZOQUINE; ORTHODIAZOQUINONE; 3-DIAZO-N-NITROSOBAMETHAN; HYDROXYPHENYL RADICAL; DMPO SPIN ADDUCT; PBN SPIN ADDUCT; DNA STRAND BREAKAGE;

D O I：

10.1016/0027-5107(92)90088-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mutagenic diazoquinone compounds p-diazoquinone (p-DQ), o-diazoquinone (o-DQ) and 3-diazo-N-nitrosobamethan (D-BM) cleaved the phosphodiester bond of lambda-DNA, PHI-X174 RFI DNA and M13mp8ss DNA. p-DQ also cleaved the phosphodiester bond of bis(p-nitrophenyl)phosphate. Thc breakage of the phosphodiester bond was inhibited by the antioxidant butyl hydroxyanisole (BHA), ethanol, the spin trapping agent DMPO, cysteine and 2-mercaptoethanol. While incubation of p-DQ and o-DQ alone gave p-hydroquinone and catechol, respectively, incubation of these compounds in the presence of BHA and ethanol gave phenol in large yields. Incubation of p-DQ and o-DQ with the spin trapping agents DMPO and PBN gave spin adducts assignable as p- and o-hydroxyphenyl adducts, respectively. The breakage of the phosphodiester bond of DNA by the diazoquinone compounds is suggested to be due to the hydroxyphenyl radicals generated during incubation.

引用

页码：105 / 114

页数：10

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