COOPERATIVE MEMBRANE INSERTION OF MAGAININ CORRELATED WITH ITS CYTOLYTIC ACTIVITY

被引：163

作者：

LUDTKE, SJ ^{[1
]}

HE, K ^{[1
]}

WU, YL ^{[1
]}

HUANG, HW ^{[1
]}

机构：

[1] RICE UNIV,DEPT PHYS,HOUSTON,TX 77251

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1190卷 / 01期

关键词：

MAGAININ; ALAMETHICIN; LIPID BILAYER; PROTEIN-LIPID INTERACTION; CYTOLYSIS;

D O I：

10.1016/0005-2736(94)90050-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using oriented circular dichroism, we have found that magainin adopts an alpha-helical conformation with two distinct orientations when interacting with a lipid bilayer. At low concentrations, magainin is adsorbed parallel to the membrane surface. However, at high concentrations, magainin is inserted into the membrane. This transition occurs at roughly the same critical concentration required for cytolytic activity, implying that-the membrane insertion is responsible for magainin's cell-lysing activity.

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收藏

页码：181 / 184

页数：4

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