CLONING OF 2 ISOZYMES OF TRICHODERMA-KONINGII GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE WITH DIFFERENT SENSITIVITY TO KONINGIC ACID

被引：8

作者：

WATANABE, H ^{[1
]}

HASUMI, K ^{[1
]}

FUKUSHIMA, Y ^{[1
]}

SAKAI, K ^{[1
]}

ENDO, A ^{[1
]}

机构：

[1] TOKYO NOKO UNIV,DEPT APPL BIOL SCI,3-5-8 SAIWAICHO,FUCHU,TOKYO 183,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1172卷 / 1-2期

关键词：

KONINGIC ACID; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ISOZYME; CLONING; NUCLEOTIDE SEQUENCING; (T-KONINGII);

D O I：

10.1016/0167-4781(93)90267-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Koningic acid inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by binding to the SH group in the active center. The fungus Trichoderma koningii, the producer of koningic acid, contains two GAPDH isozymes (GAPDHs I and II). GAPDH I is inhibited 50% by 1.1 . 10(-3) M koningic acid, while GAPDH II is inhibited 50% at 6.8 . 10(-6) M. cDNAs of the two isozymes were cloned from T. koningii and their nucleotide sequences were determined. The sequence of coding region and codon usage in both clones were compared with each other and with those of the gene for Aspergillus nidulans GAPDH (enzyme activity is inhibited 50% by 2.7 . 10(-7) M koningic acid). Results indicated that GAPDH II is more closely related to A. nidulans GAPDH than GAPDH I. All essential amino acid residues, except 174 and 181, which are implicated in catalysis and binding of NAD and substrates, were conserved among A. nidulans GAPDH and GAPDHs I and II. Residues 174 and 181 are threonine in both A. nidulans GAPDH and GAPDH II, but alanine and serine, respectively, in GAPDH I. The side-chain of alanine-174 in GAPDH I can not replace threonine-174 functionally as threonine-174 side-chain forms a hydrogen bond with the catalytically essential histidine-176.

引用

页码：43 / 48

页数：6

共 33 条

[31]

TSO JY, 1985, J BIOL CHEM, V260, P8220

[32] NUCLEOTIDE-SEQUENCE OF HAMSTER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MESSENGER-RNA [J].

VINCENT, S ;

FORT, P .

NUCLEIC ACIDS RESEARCH, 1990, 18 (10) :3054-3054

[33]

YARBROUGH PO, 1984, J BIOL CHEM, V259, P4711

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