COAXIAL CAPILLARY AND CONDUCTIVE CAPILLARY INTERFACES FOR COLLECTION OF FRACTIONS-ISOLATED BY CAPILLARY ELECTROPHORESIS

被引：15

作者：

CHIU, RW ^{[1
]}

WALKER, KL ^{[1
]}

HAGEN, JJ ^{[1
]}

MONNIG, CA ^{[1
]}

WILKINS, CL ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT CHEM,RIVERSIDE,CA 92521

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 22期

关键词：

D O I：

10.1021/ac00118a024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An instrument is described that allows the automated collection of fractions isolated by capillary electrophoresis, This instrument allows the electrical connection to be established with the separation capillary by using a coaxial capillary how cell or by treating the outer surface of the capillary with a gold-filled epoxy to allow electrophoresis, The coaxial interface is most useful when the electroosmotic now in the capillary is small, and the conductive capillary interface is favored when dilution and contamination of the sample must be minimized, Both geometries permit closely spaced fractions to be acquired with minimal cross-contamination and dilution, Sample recoveries were better than 80% and virtually independent of the chemical characteristics of the sample. Fractions isolated with this instrument were successfully analyzed by high-pressure liquid chromatography and electrospray mass spectrometry.

引用

页码：4190 / 4196

页数：7

共 25 条

[1] THE USE OF CAPILLARY ELECTROPHORESIS IN A MICROPREPARATIVE MODE - METHODS AND APPLICATIONS [J].