IONSPRAY MASS-SPECTROMETRY OF MARINE TOXINS .4. DETERMINATION OF DIARRHETIC SHELLFISH POISONING TOXINS IN MUSSEL TISSUE BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：43

作者：

PLEASANCE, S ^{[1
]}

QUILLIAM, MA ^{[1
]}

MARR, JC ^{[1
]}

机构：

[1] FENWICK LABS, HALIFAX B3H 4M2, NS, CANADA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 02期

关键词：

D O I：

10.1002/rcm.1290060210

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved liquid chromatographic/mass spectrometric (LC/MS) method utilizing gradient elution and ion-spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin-1, the principal toxins implicated in case of diarrhetic shellfish poisoning. The method was used to confirm the presence of both toxins, together with a recently identified isomer of okadaic acid, dinophysistoxin-2, in various samples of cultivated blue mussels (Mytilus edulis) from Canadian and European waters. The method provided a mass detection limit of 0.4 ng for each toxin, thus allowing detection of 40 ng per g of whole mussel tissue (or approximately 10 ng/g if only the digestive glands were used in the assay). Quantitative results obtained by LC/MS were in good agreement with those obtained by derivatization and high-performance liquid chromatography with fluorescence detection.

引用

页码：121 / 127

页数：7

共 19 条

[1] N-(9-ACRIDINYL)-BROMOACETAMIDE - A POWERFUL REAGENT FOR PHASE-TRANSFER-CATALYZED FLUORESCENCE LABELING OF CARBOXYLIC-ACIDS FOR LIQUID-CHROMATOGRAPHY
ALLENMARK, S
CHELMINSKABERTILSSON, M
THOMPSON, RA
[J]. ANALYTICAL BIOCHEMISTRY, 1990, 185 (02) : 279 - 285
[2] MASS-SPECTRAL CHARACTERISTICS OF OKADAIC ACID AND SIMPLE DERIVATIVES
BENCSATH, FA
DICKEY, RW
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1991, 5 (06) : 283 - 290
[3] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY
BRUINS, AP
COVEY, TR
HENION, JD
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (22) : 2642 - 2646
[4] Cembella A.D., 1989, Journal of Applied Phycology, V1, P307, DOI 10.1007/BF00003466
[5] IDENTIFICATION OF OKADAIC ACID FROM A CARIBBEAN DINOFLAGELLATE, PROROCENTRUM-CONCAVUM
DICKEY, RW
BOBZIN, SC
FAULKNER, DJ
BENCSATH, FA
ANDRZEJEWSKI, D
[J]. TOXICON, 1990, 28 (04) : 371 - 377
[6] HAAMER J, 1990, Journal of Shellfish Research, V9, P109
[7] REFERENCE MATERIALS FOR DOMOIC ACID, A MARINE NEUROTOXIN
HARDSTAFF, WR
JAMIESON, WD
MILLEY, JE
QUILLIAM, MA
SIM, PG
[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1990, 338 (04): : 520 - 525
[8] HU T, IN PRESS CHEM COMMUN
[9] DINOPHYSIS-ACUMINATA BLOOMS IN THE DUTCH COASTAL AREA RELATED TO DIARRHETIC MUSSEL POISONING IN THE DUTCH WADDENSEA
KAT, M
[J]. SARSIA, 1983, 68 (01): : 81 - 84
[10] FLUOROMETRIC-DETERMINATION OF DIARRHETIC SHELLFISH TOXINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
LEE, JS
YANAGI, T
KENMA, R
YASUMOTO, T
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (03): : 877 - 881

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