IONSPRAY MASS-SPECTROMETRY OF MARINE TOXINS .4. DETERMINATION OF DIARRHETIC SHELLFISH POISONING TOXINS IN MUSSEL TISSUE BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：43

作者：

PLEASANCE, S ^{[1
]}

QUILLIAM, MA ^{[1
]}

MARR, JC ^{[1
]}

机构：

[1] FENWICK LABS, HALIFAX B3H 4M2, NS, CANADA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 02期

关键词：

D O I：

10.1002/rcm.1290060210

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved liquid chromatographic/mass spectrometric (LC/MS) method utilizing gradient elution and ion-spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin-1, the principal toxins implicated in case of diarrhetic shellfish poisoning. The method was used to confirm the presence of both toxins, together with a recently identified isomer of okadaic acid, dinophysistoxin-2, in various samples of cultivated blue mussels (Mytilus edulis) from Canadian and European waters. The method provided a mass detection limit of 0.4 ng for each toxin, thus allowing detection of 40 ng per g of whole mussel tissue (or approximately 10 ng/g if only the digestive glands were used in the assay). Quantitative results obtained by LC/MS were in good agreement with those obtained by derivatization and high-performance liquid chromatography with fluorescence detection.

引用

页码：121 / 127

页数：7

共 19 条

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