FURTHER-STUDIES OF THE CULTURE OF MOUSE HYBRIDOMAS IN AN AGITATED BIOREACTOR WITH AND WITHOUT CONTINUOUS SPARGING

被引:66
作者
OH, SKW [1 ]
NIENOW, AW [1 ]
ALRUBEAI, M [1 ]
EMERY, AN [1 ]
机构
[1] UNIV BIRMINGHAM,SCH CHEM ENGN,SERC,CTR BIOCHEM ENGN,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
关键词
CELL CULTURE; DISSOLVED-O2; MAB PRODUCTION; AGITATION; AERATION; BUBBLE-CELL INTERACTIONS;
D O I
10.1016/0168-1656(92)90144-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
TB/C3 mouse hybridoma cells have been grown at 2 controlled dO2 conditions by headspace and sparged oxygenation. Also a variety of sparging rates and sparger sizes and positions have been employed. Headspace oxygenation at dO2 levels from 5% to 100% of saturation give essentially the same performance as controls. Sparging is generally damaging to cells, the extent of damage decreasing with reduced sparging rate until at below about 0.02 vvm results equivalent to the unsparged conditions are obtained. Damage is clearly linked with bubble-cell interactions at the air-medium interface where bubbles bursting in clusters and of a size less than 5 mm appear to be the most lethal. When the interaction of air sparging with the agitator flow leads to an increase in the number of smaller bubbles and cluster bursts, cell damage is further increased. Pluronic F-68 reduces damage very significantly. Biological aspects are briefly discussed in the light of various biological tests. The practical implications of this work for large scale, free suspension cell culture are outlined.
引用
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页码:245 / 270
页数:26
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