P-31 NMR CHARACTERIZATION OF TERMINAL PHOSPHATES INDUCED ON DNA BY THE ARTIFICIAL NUCLEASE MN-TMPYP/KHSO5 IN COMPARISON WITH DNASES-I AND DNASES-II

被引：23

作者：

GASMI, G ^{[1
]}

PASDELOUP, M ^{[1
]}

PRATVIEL, G ^{[1
]}

PITIE, M ^{[1
]}

BERNADOU, J ^{[1
]}

MEUNIER, B ^{[1
]}

机构：

[1] CNRS, CHIM COORDINAT LAB, 205 ROUTE NARBONNE, F-31077 TOULOUSE, FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 11期

关键词：

D O I：

10.1093/nar/19.11.2835

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.

引用

页码：2835 / 2839

页数：5

共 31 条

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