DIRECT IDENTIFICATION OF TRYPANOSOMA-CRUZI NATURAL CLONES IN VECTORS AND MAMMALIAN HOSTS BY POLYMERASE CHAIN-REACTION AMPLIFICATION

被引：60

作者：

BRENIERE, SF

BOSSENO, MF

REVOLLO, S

RIVERA, MT

CARLIER, Y

TIBAYRENC, M

机构：

[1] INST BOLIVIANO TECHNOL AGROPECUARIA,DEPT PARASITOL,LA PAZ,BOLIVIA

[2] UNIV LIBRE BRUXELLES,PARASITOL LAB,B-1050 BRUSSELS,BELGIUM

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1992年 / 46卷 / 03期

关键词：

D O I：

10.4269/ajtmh.1992.46.335

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The polymerase chain reaction was used to amplify the highly variable region of the kinetoplast minicircle of Trypanosoma cruzi directly in biological samples (feces of infected Triatomine bugs, blood samples of experimentally infected mice, and artificially infected human blood samples). Hybridization of the amplified DNAs with reference stocks representing different genotypes (natural clones) enabled us to characterize the stocks infecting the biological samples under study. The main interest of this new approach is the diagnosis of T. cruzi infection and simultaneous direct identification of the different natural clones circulating in vectors and mammalian blood without isolation of the stocks. The suitability of this technique for epidemiologic studies is also discussed.

引用

页码：335 / 341

页数：7

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[11] BIOLOGICAL CHARACTERIZATION OF TRYPANOSOMA-CRUZI ZYMODEMES - INVITRO DIFFERENTIATION OF EPIMASTIGOTES AND INFECTIVITY OF CULTURE METACYCLIC TRYPOMASTIGOTES TO MICE [J].