SENSITIVE FLUORESCENCE DETECTION OF ROBENIDINE BY DERIVATIZATION WITH DANSYL CHLORIDE AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10

作者：

COHEN, H

ARMSTRONG, F

CAMPBELL, H

机构：

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 694卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)01096-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A normal-phase high-performance liquid chromatographic procedure is presented for the determination of robenidine following derivatization with dansyl chloride. The derivatization, separation and detection conditions were optimized. The dansyl derivative is first purified on a silica gel cartridge followed by HPLC analysis on a silica gel column. The eluate is monitored by fluorescence detection at 320 nm excitation and 485 nm emission wavelength. The lower limit of detection of robenidine is 0.4 mu g/ml.

引用

页码：407 / 413

页数：7

共 13 条

[1]

AL-SHAWABKEH K, 1990, Dirasat Series B Pure and Applied Sciences, V17, P86

[2] SIMPLE DETERMINATION OF COCCIDIOSTAT ROBENIDINE IN POULTRY FEED [J].