CLONING AND SEQUENCING OF THE RAT CDNA-ENCODING THE MITOCHONDRIAL 2-OXOGLUTARATE CARRIER PROTEIN

被引：13

作者：

DOLCE, V

MESSINA, A

CAMBRIA, A

PALMIERI, F

机构：

[1] UNIV BARI,DEPT PHARMACOBIOL,BIOCHEM & MOLEC BIOL LAB,BARI,ITALY

[2] UNIV CATANIA,INST BIOCHEM & PHARMACOL SCI,CATANIA,ITALY

来源：

DNA SEQUENCE | 1994年 / 5卷 / 02期

关键词：

CDNA CLONE; MITOCHONDRIAL; 2-OXOGLUTARATE CARRIER; SEQUENCE;

D O I：

10.3109/10425179409039711

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 2-oxoglutarate carrier protein (OGCP) catalyzes the transport of the 2-oxoglutarate into the mitochondrial matrix by an electroneutral exchange for malate or some other dicarboxylic acids. Using primers based on the bovine heart cDNA sequence, overlapping cDNA clones encoding the rat OGCP were isolated from total rat heart poly(A(+)) cDNA. The entire rat cDNA is 1149 bp in length with 5' and 3' untranslated regions of 41 and 163 bp, respectively. The open reading frame encodes a protein consisting of 314 amino acids. The amino acid sequence of the rat 2-oxoglutarate carrier is 97% identical to that of the 2-oxoglutarate from cow and human. By Northern blot analysis, hybridizing transcripts were found in rat heart, liver and brain.

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页码：103 / 109

页数：7

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