CLONING AND SEQUENCING OF THE RAT CDNA-ENCODING THE MITOCHONDRIAL 2-OXOGLUTARATE CARRIER PROTEIN

被引:13
作者
DOLCE, V
MESSINA, A
CAMBRIA, A
PALMIERI, F
机构
[1] UNIV BARI,DEPT PHARMACOBIOL,BIOCHEM & MOLEC BIOL LAB,BARI,ITALY
[2] UNIV CATANIA,INST BIOCHEM & PHARMACOL SCI,CATANIA,ITALY
来源
DNA SEQUENCE | 1994年 / 5卷 / 02期
关键词
CDNA CLONE; MITOCHONDRIAL; 2-OXOGLUTARATE CARRIER; SEQUENCE;
D O I
10.3109/10425179409039711
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 2-oxoglutarate carrier protein (OGCP) catalyzes the transport of the 2-oxoglutarate into the mitochondrial matrix by an electroneutral exchange for malate or some other dicarboxylic acids. Using primers based on the bovine heart cDNA sequence, overlapping cDNA clones encoding the rat OGCP were isolated from total rat heart poly(A(+)) cDNA. The entire rat cDNA is 1149 bp in length with 5' and 3' untranslated regions of 41 and 163 bp, respectively. The open reading frame encodes a protein consisting of 314 amino acids. The amino acid sequence of the rat 2-oxoglutarate carrier is 97% identical to that of the 2-oxoglutarate from cow and human. By Northern blot analysis, hybridizing transcripts were found in rat heart, liver and brain.
引用
收藏
页码:103 / 109
页数:7
相关论文
共 16 条
[1]   BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].
BIGGIN, MD ;
GIBSON, TJ ;
HONG, GF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965
[2]   PURIFICATION AND RECONSTITUTION OF 2 ANION CARRIERS FROM RAT-LIVER MITOCHONDRIA - THE DICARBOXYLATE AND THE 2-OXOGLUTARATE CARRIER [J].
BISACCIA, F ;
INDIVERI, C ;
PALMIERI, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 933 (02) :229-240
[3]   PURIFICATION OF RECONSTITUTIVELY ACTIVE ALPHA-OXOGLUTARATE CARRIER FROM PIG-HEART MITOCHONDRIA [J].
BISACCIA, F ;
INDIVERI, C ;
PALMIERE, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 810 (03) :362-369
[4]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[5]  
Iacobazzi V, 1992, DNA Seq, V3, P79, DOI 10.3109/10425179209034000
[6]   METABOLIC SIGNIFICANCE OF ANION TRANSPORT IN MITOCHONDRIA [J].
MEIJER, AJ ;
VANDAM, K .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 346 (3-4) :213-244
[7]   THYROID-HORMONE INCREASES GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE-EXPRESSION IN RAT-LIVER [J].
NESS, GC ;
PENDLETON, LC .
FEBS LETTERS, 1991, 288 (1-2) :21-22
[8]   KINETICS AND SPECIFICITY OF OXOGLUTARATE CARRIER IN RAT-LIVER MITOCHONDRIA [J].
PALMIERI, F ;
QUAGLIAR.E ;
KLINGENB.M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 29 (03) :408-&
[9]   2 BOVINE GENES FOR MITOCHONDRIAL ADP ATP TRANSLOCASE EXPRESSED DIFFERENTLY IN VARIOUS TISSUES [J].
POWELL, SJ ;
MEDD, SM ;
RUNSWICK, MJ ;
WALKER, JE .
BIOCHEMISTRY, 1989, 28 (02) :866-873
[10]   SEQUENCE OF THE BOVINE 2-OXOGLUTARATE MALATE CARRIER PROTEIN - STRUCTURAL RELATIONSHIP TO OTHER MITOCHONDRIAL TRANSPORT PROTEINS [J].
RUNSWICK, MJ ;
WALKER, JE ;
BISACCIA, F ;
IACOBAZZI, V ;
PALMIERI, F .
BIOCHEMISTRY, 1990, 29 (50) :11033-11040