4-(17-TETRABENZO[A,C,G,I]FLUORENYLMETHYL)-4',4''-DIMETHOXYTRITYL CHLORIDE - A HYDROPHOBIC 5'-PROTECTING GROUP FOR THE SEPARATION OF SYNTHETIC OLIGONUCLEOTIDES

被引:23
作者
RAMAGE, R
WAHL, FO
机构
[1] Department of Chemistry, University of Edinburgh, Edinburgh, EH9 3JJ Scotland, West Mains Road
关键词
RP-HPLC; 4-(17-TETRABENZO[A; C; G; I]FLUORENYLMETHYL)-4'; 4''-DIMETHOXYRITYL CHLORIDE; TBF-DMTR; DMTR; PROTECTING GROUP; OLIGONUCLEOTIDE SYNTHESIS;
D O I
10.1016/S0040-4039(00)61618-0
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A new highly hydrophobic 5'-hydroxyl protecting group (Tbf-DMTr) has been designed for the purification of synthetic oligonucleotides. Tbf-DMTr-oligonucleotides are strongly retained on RP-HPLC allowing a facile separation from truncated sequences. Subsequently the group can be removed in acidic conditions. The ultra-violet properties of Tbf-DMTr enable easy detection. The synthesis and purification of long oligonucleotides (> 100 mer) is envisaged.
引用
收藏
页码:7133 / 7136
页数:4
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