RAPID CHARACTERIZATION OF MYCOBACTERIUM-FORTUITUM-CHELONEI COMPLEX BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF RIBOSOMAL-RNA GENES

被引：4

作者：

KANAUJIA, GV ^{[1
]}

KATOCH, VM ^{[1
]}

SHIVANNAVAR, CT ^{[1
]}

SHARMA, VD ^{[1
]}

PATIL, MA ^{[1
]}

机构：

[1] ICMR,CENT JALMA INST LEPROSY,AGRA 282001,UTTAR PRADESH,INDIA

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 77卷 / 2-3期

关键词：

MYCOBACTERIUM; RIBOSOMAL-RNA GENE; RESTRICTION ENDONUCLEASE; TAXONOMY;

D O I：

10.1016/0378-1097(91)90552-L

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using labelled, gamma-P-32 rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with gamma-P-32-labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum-chelonei complex.

引用

页码：205 / 208

页数：4

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