FORMATION OF PLANAR SOLVENT-FREE PHOSPHOLIPID-BILAYERS BY LANGMUIR-BLODGETT TRANSFER OF MONOLAYERS TO MICROMACHINED APERTURES IN SILICON

被引：27

作者：

OSBORN, TD ^{[1
]}

YAGER, P ^{[1
]}

机构：

[1] UNIV WASHINGTON,CTR BIOENGN,MOLEC BIOENGN PROGRAM,SEATTLE,WA 98195

来源：

LANGMUIR | 1995年 / 11卷 / 01期

关键词：

D O I：

10.1021/la00001a003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Solvent-free lipid bilayers were reproducibly formed across 100 mu m apertures in a hydrophilic micromachined silicon support. The bilayers were assembled from mixed phosphatidylcholine/cholesterol monolayers at the air-water interface by the Langmuir-Blodgett method. The presence and longevity of the bilayers were determined by fluorescence microscopy. The bilayer seal impedance was also measured. Such a mass producible device for supporting lipid bilayers is of potential value for biosensor development, for receptor and drug assays, and for biophysical studies of ion channels.

引用

页码：8 / 12

页数：5

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