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INOCULATION OF SUGAR-BEET PROTOPLASTS WITH BEET NECROTIC YELLOW VEIN VIRUS-PARTICLES BY MILD SONICATION
被引:10
作者
:
JOERSBO, M
论文数:
0
引用数:
0
h-index:
0
机构:
DANISCO A/S, Biotechnology Research Division, Copenhagen
JOERSBO, M
BRUNSTEDT, J
论文数:
0
引用数:
0
h-index:
0
机构:
DANISCO A/S, Biotechnology Research Division, Copenhagen
BRUNSTEDT, J
机构
:
[1]
DANISCO A/S, Biotechnology Research Division, Copenhagen
来源
:
JOURNAL OF VIROLOGICAL METHODS
|
1990年
/ 29卷
/ 01期
关键词
:
Beet necrotic yellow vein virus;
Protoplast;
Sugar beet;
Ultrasound;
Virus infection;
D O I
:
10.1016/0166-0934(90)90008-4
中图分类号
:
Q5 [生物化学];
学科分类号
:
071010 ;
081704 ;
摘要
:
A novel procedure employing mild sonication for in vitro inoculation of plant protoplasts with virus particles has been established. Sugar beet (Beta vulgaris L.) protoplasts were briefly exposed to 20 kHz ultrasound in the presence of beet necrotic yellow vein virus (BNYVV) particles. The accumulation of BNYVV coat protein was analyzed by an enzyme-linked immunosorbent assay. The infection was detectable after 16 h and reached maximum 3-3 1 2 days after inoculation. Maximum levels of BNYVV coat protein in inoculated protoplasts were obtained by sonication for 500-600 ms at 45-60 W. This reduced the viability to 15-30%. The efficiency of the inoculation increased with the concentration of BNYVV particles up to 28 μg/ml. Infection was found to be optimal when the BNYVV particles were added just before sonication but low levels of infection could be obtained by addition of virus particles up to 60 min after sonication. © 1990.
引用
收藏
页码:63 / 69
页数:7
相关论文
共 16 条
[11]
COMPOSITION AND STRUCTURE OF BEET NECROTIC YELLOW VEIN VIRUS
PUTZ, C
论文数:
0
引用数:
0
h-index:
0
机构:
INRA, PATHOL VEGETALE STN, F-68021 COLMAR, FRANCE
INRA, PATHOL VEGETALE STN, F-68021 COLMAR, FRANCE
PUTZ, C
[J].
JOURNAL OF GENERAL VIROLOGY,
1977,
35
(MAY)
: 397
-
401
[12]
LIPOSOME-MEDIATED INFECTION OF TURNIP PROTOPLASTS WITH TURNIP ROSETTE VIRUS AND RNA
ROLLO, F
论文数:
0
引用数:
0
h-index:
0
机构:
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
ROLLO, F
HULL, R
论文数:
0
引用数:
0
h-index:
0
机构:
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
HULL, R
[J].
JOURNAL OF GENERAL VIROLOGY,
1982,
60
(JUN)
: 359
-
363
[13]
2 PURIFIED RNAS OF SOIL-BORNE WHEAT MOSAIC-VIRUS ARE NEEDED FOR INFECTION
SHIRAKO, Y
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV NEBRASKA,USDA,ARS,LINCOLN,NE 68583
SHIRAKO, Y
BRAKKE, MK
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV NEBRASKA,USDA,ARS,LINCOLN,NE 68583
BRAKKE, MK
[J].
JOURNAL OF GENERAL VIROLOGY,
1984,
65
(JAN)
: 119
-
127
[14]
CALLUS FORMATION FROM PROTOPLASTS OF A SUGARBEET CELL-SUSPENSION CULTURE
SZABADOS, L
论文数:
0
引用数:
0
h-index:
0
机构:
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SZABADOS, L
GAGGERO, C
论文数:
0
引用数:
0
h-index:
0
机构:
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
GAGGERO, C
[J].
PLANT CELL REPORTS,
1985,
4
(04)
: 195
-
198
[15]
INOCULATION OF PROTOPLASTS WITH VIRUSES BY ELECTROPORATION
WATTS, JW
论文数:
0
引用数:
0
h-index:
0
WATTS, JW
KING, JM
论文数:
0
引用数:
0
h-index:
0
KING, JM
STACEY, NJ
论文数:
0
引用数:
0
h-index:
0
STACEY, NJ
[J].
VIROLOGY,
1987,
157
(01)
: 40
-
46
[16]
WIDHOLM JM, 1972, STAIN TECHNOL, V47, P189
←
1
2
→
共 16 条
[11]
COMPOSITION AND STRUCTURE OF BEET NECROTIC YELLOW VEIN VIRUS
PUTZ, C
论文数:
0
引用数:
0
h-index:
0
机构:
INRA, PATHOL VEGETALE STN, F-68021 COLMAR, FRANCE
INRA, PATHOL VEGETALE STN, F-68021 COLMAR, FRANCE
PUTZ, C
[J].
JOURNAL OF GENERAL VIROLOGY,
1977,
35
(MAY)
: 397
-
401
[12]
LIPOSOME-MEDIATED INFECTION OF TURNIP PROTOPLASTS WITH TURNIP ROSETTE VIRUS AND RNA
ROLLO, F
论文数:
0
引用数:
0
h-index:
0
机构:
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
ROLLO, F
HULL, R
论文数:
0
引用数:
0
h-index:
0
机构:
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
JOHN INNES INST,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
HULL, R
[J].
JOURNAL OF GENERAL VIROLOGY,
1982,
60
(JUN)
: 359
-
363
[13]
2 PURIFIED RNAS OF SOIL-BORNE WHEAT MOSAIC-VIRUS ARE NEEDED FOR INFECTION
SHIRAKO, Y
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV NEBRASKA,USDA,ARS,LINCOLN,NE 68583
SHIRAKO, Y
BRAKKE, MK
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV NEBRASKA,USDA,ARS,LINCOLN,NE 68583
BRAKKE, MK
[J].
JOURNAL OF GENERAL VIROLOGY,
1984,
65
(JAN)
: 119
-
127
[14]
CALLUS FORMATION FROM PROTOPLASTS OF A SUGARBEET CELL-SUSPENSION CULTURE
SZABADOS, L
论文数:
0
引用数:
0
h-index:
0
机构:
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SZABADOS, L
GAGGERO, C
论文数:
0
引用数:
0
h-index:
0
机构:
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
SUGARBEET RES INST,H-9463 SOPRONHORPACS,HUNGARY
GAGGERO, C
[J].
PLANT CELL REPORTS,
1985,
4
(04)
: 195
-
198
[15]
INOCULATION OF PROTOPLASTS WITH VIRUSES BY ELECTROPORATION
WATTS, JW
论文数:
0
引用数:
0
h-index:
0
WATTS, JW
KING, JM
论文数:
0
引用数:
0
h-index:
0
KING, JM
STACEY, NJ
论文数:
0
引用数:
0
h-index:
0
STACEY, NJ
[J].
VIROLOGY,
1987,
157
(01)
: 40
-
46
[16]
WIDHOLM JM, 1972, STAIN TECHNOL, V47, P189
←
1
2
→