PEPTIDES WHICH BIND TO E-SELECTIN AND BLOCK NEUTROPHIL ADHESION

被引：136

作者：

MARTENS, CL ^{[1
]}

CWIRLA, SE ^{[1
]}

LEE, RYW ^{[1
]}

WHITEHORN, E ^{[1
]}

CHEN, EYF ^{[1
]}

BAKKER, A ^{[1
]}

MARTIN, EL ^{[1
]}

WAGSTROM, C ^{[1
]}

GOPALAN, P ^{[1
]}

SMITH, CW ^{[1
]}

TATE, E ^{[1
]}

KOLLER, KJ ^{[1
]}

SCHATZ, PJ ^{[1
]}

DOWER, WJ ^{[1
]}

BARRETT, RW ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT LEUKOCYTE BIOL,HOUSTON,TX 77030

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 36期

关键词：

D O I：

10.1074/jbc.270.36.21129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

E-selectin is an inducible cell adhesion molecule which mediates rolling of neutrophils on the endothelium, an early event in the development of an inflammatory response, inhibition of selectin-mediated rolling is a possible means for controlling inflammation-induced diseases, and several classes of compounds have been tested for this use. We describe here the use of recombinant peptide library screening for identification and optimization of novel ligands which bind to E-selectin. Several of these peptides bind with K-d values in the low nanomolar range and block E-selectin-mediated adhesion of neutrophils in static and flow cell assays. Administration of the peptide to mice undergoing an acute inflammatory response reduced the extent of neutrophil transmigration to the site of inflammation, demonstrating the utility of this compound as a potential therapeutic. The identification of a peptide ligand for E-selectin suggests that the complete natural ligand for this adhesion molecule may include protein as well as carbohydrate moieties.

引用

页码：21129 / 21136

页数：8

共 82 条

[31] AN ENDOTHELIAL LIGAND FOR L-SELECTIN IS A NOVEL MUCIN-LIKE MOLECULE [J].