APPLICATION OF A DNA-DNA HYBRIDIZATION METHOD FOR DETECTION OF CAMPYLOBACTER-JEJUNI IN CHICKEN FECES

被引：8

作者：

CHUMA, T ^{[1
]}

YAMADA, T ^{[1
]}

OKAMOTO, K ^{[1
]}

YUGI, H ^{[1
]}

OHYA, T ^{[1
]}

机构：

[1] NATL INST ANIM HLTH,KYUSHU BRANCH LAB,KAGOSHIMA,KAGOSHIMA 89101,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1993年 / 55卷 / 06期

关键词：

CAMPYLOBACTER JEJUNI; CHICKEN; DNA HYBRIDIZATION;

D O I：

10.1292/jvms.55.1027

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A direct colony hybridization method was used for the detection of Campylobacter jejuni in chicken feces. The biotin-labeled DNA prepared from the whole genome DNA of C. jejuni subsp. jejuni ATCC 33560 reacted well with homologous DNA and slightly with C. coli DNA. The method with the probe was found to be sensitive enough to detect a small number (10(2) CFU/g) of C. jejuni in chicken feces which contained a large number of background flora. It was suggested that this simple and sensitive method was useful for a wide survey of C. jejuni.

引用

页码：1027 / 1029

页数：3

共 28 条

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