IDENTIFICATION OF THE PROTEIN AND CDNA OF THE CARDIAC NA+/H+ EXCHANGER

被引：68

作者：

FLIEGEL, L ^{[1
]}

SARDET, C ^{[1
]}

POUYSSEGUR, J ^{[1
]}

BARR, A ^{[1
]}

机构：

[1] UNIV NICE, CNRS, CTR BIOCHIM, F-06034 NICE, FRANCE

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

NA+/H+ EXCHANGER; SARCOLEMMA; CDNA CLONING;

D O I：

10.1016/0014-5793(91)80241-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the myocardial form of the Na+/H+ exchanger. A partial length cDNA clone was isolated from a rabbit cardiac library and it encoded for a Na+/H+ exchange protein. In comparison with the human Na+/H+ exchanger, the sequence of the 5' end of the cDNA was highly conserved, much more than the 3' region, while the deduced amino acid sequence was also highly conserved. To further characterize the myocardial Na+/H+ exchange protein, we examined Western blots of isolated sarcolemma with antibody produced against a fusion protein of the Na+/H+ exchanger. The antibodies reacted with a sarcolemma protein of 50 kDa and with a protein of 70 kDa. The results show that the rabbit myocardium does possess a Na+/H+ exchanger protein homologous to the known human Na+/H+ exchanger.

引用

页码：25 / 29

页数：5

共 30 条

[11] INFLUENCE OF INTRACELLULAR ACIDOSIS ON CONTRACTILE FUNCTION IN THE WORKING RAT-HEART [J].