PHOSPHATE DETERMINATION IN FOODSTUFFS USING A PLANT-TISSUE ELECTRODE

被引：16

作者：

CAMPANELLA, L ^{[1
]}

CORDATORE, M ^{[1
]}

MAZZEI, F ^{[1
]}

TOMASSETTI, M ^{[1
]}

VOLPE, G ^{[1
]}

机构：

[1] CNR, SSPS, ROME, ITALY

来源：

FOOD CHEMISTRY | 1992年 / 44卷 / 04期

关键词：

D O I：

10.1016/0308-8146(92)90053-5

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

It is well known that phosphate concentration can be determined from the inhibition by this species of acid phosphatase activity. A suitable plant tissue electrode for the analysis of inorganic phosphate was developed by adding the enzyme glucose oxidase to a slice of Solanum tuberosum containing the acid phosphatase enzyme and using an amperometric Clark electrode as indicating sensor. The biosensor was fully characterized and used for phosphate determination in authentic matrices such as fresh and powdered milk. wine and tomato puree. The results obtained were compared with those obtained using Bartlett's spectrometric method.

引用

页码：291 / 297

页数：7

共 7 条

[1] BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[2] DETERMINATION OF INORGANIC-PHOSPHATE IN DRUG FORMULATIONS AND BIOLOGICAL-FLUIDS USING A PLANT-TISSUE ELECTRODE
CAMPANELLA, L
CORDATORE, M
MAZZEI, F
TOMASSETTI, M
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1990, 8 (8-12) : 711 - 716
[3] Fiske CH, 1925, J BIOL CHEM, V66, P375
[4] PHOSPHATE-SELECTIVE ELECTRODE BASED ON IMMOBILIZED ALKALINE-PHOSPHATASE AND GLUCOSE OXIDASE
GUILBAULT, GG
NANJO, M
[J]. ANALYTICA CHIMICA ACTA, 1975, 78 (01) : 69 - 80
[5] HYBRID ELECTRODE FOR THE DETERMINATION OF PHOSPHATES AND POLYPHOSPHATES - APPLICATION TO ENVIRONMENTAL-PROBLEMS
LINDERS, CR
VINCKE, BJ
PATRIARCHE, GJ
[J]. ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1985, 18 (17): : 2195 - 2208
[6] DETERMINATION OF FREE AND TOTAL CHOLESTEROL IN HUMAN BILE SAMPLES USING AN ENZYME ELECTRODE
MASCINI, M
TOMASSETTI, M
IANNELLO, M
[J]. CLINICA CHIMICA ACTA, 1983, 132 (01) : 7 - 15
[7] PLANT-TISSUE HYDRID ELECTRODE FOR DETERMINATION OF PHOSPHATE AND FLUORIDE
SCHUBERT, F
RENNEBERG, R
SCHELLER, FW
KIRSTEIN, L
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (09) : 1677 - 1682

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