CHROMATOGRAPHIC AND ANTIGENIC PROPERTIES OF ECHINOCOCCUS-GRANULOSUS HYDATID CYST-DERIVED GLYCOLIPIDS

被引:20
作者
DENNIS, RD
BAUMEISTER, S
IRMER, G
GASSER, RB
GEYER, E
机构
[1] PHILIPPS UNIV, FACHBEREICH HUMANMED, INST PHYSIOL CHEM, MARBURG, GERMANY
[2] UNIV MELBOURNE, SCH VET SCI, MELBOURNE, VIC, AUSTRALIA
关键词
ECHINOCOCCUS GRANULOSUS METACESTODE GLYCOLIPIDS; HPTLC-IMMUNOSTAINING; ELISA; DIFFERENTIAL SERO-DIAGNOSIS; NEOGALA-SERIES GLYCOSPHINGOLIPIDS; P1-ACTIVITY;
D O I
10.1111/j.1365-3024.1993.tb00582.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The neutral and acidic fraction glycolipids of Echinococcus granulosus metacestode tissue compartments were isolated, defined by their chromatographic and antigenic properties, and assessed as to their efficacy as antigens in the serodiagnosis of human hepatic cystic and alveolar echinococcosis, and other helminthiases. Analyses wet e accomplished by thin-layer chromatography immunostaining and ELISA. The neutral glycolipid fraction's major carbohydrate epitope was the same as or very similar to that of Taenia crassiceps neutral glyco(sphingo)lipids, as represented by the 'neogala'-series cove structure. The blood group-active, carbohydrate epitope P1 was expressed by a number of neutral fraction glycolipid component bands. The reverse-phase, thin-layer chromatography-isolated neutral fraction glycolipid component, designated Agl, was efficient in the serological discrimination of cystic echinococcosis medium to high-titled sera. Agl did not specifically discriminate low-titred sera, i.e., other human helminthiases. The detected sialic acid residues of the acidic fraction glycolipids, on enzymatic cleavage, were identified as N-acylneuraminic acid and terminal. The acidic fraction glycolipids exhibited the paradox of only chemically minor components being antigenic towards cystic and alveolar echinococcosis infection sera. The combined acidic fraction glycolipid components Ra and Rx were capable of serological discrimination between cystic echinococcosis, alveolar echinococcosis and other helminthiases.
引用
收藏
页码:669 / 681
页数:13
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