EFFECT OF MALATHION ON BUFO-ARENARUM HENSEL DEVELOPMENT .1. ESTERASE INHIBITION AND RECOVERY

被引：17

作者：

DECASTRO, ACC ^{[1
]}

ROSENBAUM, EA ^{[1
]}

DEDANGELO, AMP ^{[1
]}

机构：

[1] UNIV NACL COMAHUE,FAC INGN,LIBIQUIMA,BUENOS AIRES 1400,RA-8300 NEUQUEN,ARGENTINA

来源：

BIOCHEMICAL PHARMACOLOGY | 1991年 / 41卷 / 04期

关键词：

D O I：

10.1016/0006-2952(91)90619-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Newly fertilized Bufo arenarum Hensel embryos were exposed continuously or for a brief period (72-120 hr) to malathion (44 ppm) and then resuspended in amphibian Ringer's solution. Continuous exposure depressed acetylcholinesterase (EC 3.1.1.7), butyrylcholinesterase (EC 3.1.1.8) and carboxylesterase (EC 3.1.1.1) activities. The activities of the three enzymes in embryos treated for 72 hr recovered after a delay of 24 hr, but these enzymes showed different rates of recovery in embryos treated for 120 hr. Acrylamide disc electrophoresis showed several bands of esterase activity in control embryos. Continuous exposure to malathion abolished all esterase activity within 48 hr, but if the exposure continued new bands of esterase activity appeared at 120 hr of exposure. The zymograms of embryos exposed for 72 or 120 hr to malathion and then transferred to uncontaminated medium for 120 hr were similar to that of control embryos.

引用

页码：491 / 495

页数：5

共 24 条

[11] ON ROLE OF SEROTONIN AND ACETYLCHOLINE IN SEA URCHIN MORPHOGENESIS [J].

GUSTAFSON, T ;

TONEBY, M .

EXPERIMENTAL CELL RESEARCH, 1970, 62 (01) :102-+

[12]

KOELLE GB, 1963, HDB EXPT PHARM CHOLY

[13] COMPARATIVE LOCALIZATION OF ACETYLCHOLINESTERASE AND PSEUDOCHOLINESTERASE DURING MORPHOGENESIS OF THE CHICKEN BRAIN [J].

LAYER, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (20) :6413-6417

[14]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[15] CHEMICAL MESSENGERS IN DEVELOPMENT - HYPOTHESIS [J].

MCMAHON, D .

SCIENCE, 1974, 185 (4156) :1012-1021

[16]

MICHALEK H, 1981, NEUROBEH TOXICOL TER, V3, P303

[17]

OBRIEN RD, 1976, INSECTICIDE BIOCH PH, P271, DOI DOI 10.1007/978-1-4899-2212-0_7

[18]

OZAKI H, 1974, DEV GROWTH DIFFER, V16, P267

[19]

OZAKI H, 1976, DEV GROWTH DIFFER, V18, P245

[20]

PANT R, 1978, CURR SCI INDIA, V47, P445

← 1 2 3 →