SYNTHESIS AND BIOPHYSICAL AND BIOLOGICAL PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 2-AZA-2'-DEOXYINOSINE

被引：14

作者：

ACEDO, M

DECLERCQ, E

ERITJA, R

机构：

[1] CSIC,CID,DEPT MOLEC GENET,E-08034 BARCELONA,SPAIN

[2] CATHOLIC UNIV LEUVEN,REGA INST MED RES,B-3000 LOUVAIN,BELGIUM

[3] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1995年 / 60卷 / 20期

关键词：

D O I：

10.1021/jo00125a009

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The preparation of oligonucleotides containing 2-aza-2'-deoxyinosine is described. Protection of the 2-azahypoxanthine moiety with the photolabile 2-nitrobenzyl group enabled us to obtain the phosphoramidite derivative and oligonucleotides containing protected 2-aza-2'-deoxyinosine. After purification, photolysis of the oligonucleotides containing the protected analogue provided the desired oligonucleotides in good yields. Melting curves of duplexes containing 2-azahypoxanthine paired with the four natural bases at pH 6 and pH 8 proved that 2-azahypoxanthine base pairs were less stable than perfectly matched duplexes but showed little variation among different bases.

引用

页码：6262 / 6269

页数：8

共 46 条

[41] PHOSPHORAMIDITES OF BASE-MODIFIED 2-STAR-DEOXYINOSINE ISOSTERES AND SOLID-PHASE SYNTHESIS OF D(GCI-STAR-CGC) OLIGOMERS CONTAINING AN AMBIGUOUS BASE [J].