MUSCIMOL PREVENTS NEURONAL INJURY-INDUCED BY NMDA

被引：41

作者：

OHKUMA, S ^{[1
]}

CHEN, SH ^{[1
]}

KATSURA, M ^{[1
]}

CHEN, DZ ^{[1
]}

KURIYAMA, K ^{[1
]}

机构：

[1] KYOTO PREFECTURAL UNIV MED,DEPT PHARMACOL,KAMIKYO KU,KYOTO 602,JAPAN

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1994年 / 64卷 / 02期

关键词：

GABA(A)-RECEPTOR AGONIST; NMDA RECEPTOR; NEURONAL INJURY;

D O I：

10.1254/jjp.64.125

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of muscimol on N-methyl-D-aspartate (NMDA)-induced injury of primary cultured cerebral cortical neurons was examined. NMDA induced a dose-dependent leakage of LDH activity, which was significantly inhibited by (+/-)-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]cyclopentan-5,10-imine (MK-801). Muscimol significantly reduced the NMDA-induced increase of lactic dehydrogenase (LDH) leakage, and bicuculline abolished this protective effect of muscimol. Similarly, muscimol reduced the NMDA-induced increase in trypan blue staining of the cells, and bicuculline suppressed this inhibitory action of muscimol. These results suggest that GABAA-receptor stimulation exerts a protective action against the neuronal injury induced by NMDA-receptor activation.

引用

页码：125 / 128

页数：4

共 19 条

[1]

AANONSEN LM, 1989, J PHARMACOL EXP THER, V248, P1034

[2] BIOLOGICAL DIFFERENCES BETWEEN ISCHEMIA, HYPOGLYCEMIA, AND EPILEPSY [J].

AUER, RN ;

SIESJO, BK .

ANNALS OF NEUROLOGY, 1988, 24 (06) :699-707

[3] COLORIMETRIC MEASUREMENT OF LACTIC DEHYDROGENASE ACTIVITY OF BODY FLUIDS [J].

CABAUD, PG ;

WROBLEWSKI, F .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1958, 30 (03) :234-236

[4] GLUTAMATE NEUROTOXICITY AND DISEASES OF THE NERVOUS-SYSTEM [J].

CHOI, DW .

NEURON, 1988, 1 (08) :623-634

[5]

CHOI DW, 1988, J NEUROSCI, V8, P185

[6] GABA ACCELERATES EXCITOTOXIC CELL-DEATH IN CORTICAL CULTURES - PROTECTION BY BLOCKERS OF GABA-GATED CHLORIDE CHANNELS [J].

ERDO, SL ;

MICHLER, A ;

WOLFF, JR .

BRAIN RESEARCH, 1991, 542 (02) :254-258

[7] NOVEL GLUTAMATE RECEPTOR ANTAGONISTS SELECTIVELY PROTECT AGAINST KAINIC ACID NEUROTOXICITY IN CULTURED CEREBRAL-CORTEX NEURONS [J].

FRANDSEN, A ;

KROGSGAARDLARSEN, P ;

SCHOUSBOE, A .

JOURNAL OF NEUROCHEMISTRY, 1990, 55 (05) :1821-1823

[8] PROTECTIVE EFFECT OF SYNAPTIC INHIBITION DURING CEREBRAL-ISCHEMIA IN RATS AND RABBITS [J].

LYDEN, PD ;

HEDGES, B .

STROKE, 1992, 23 (10) :1463-1469

[9] EXCITATORY AMINO-ACID NEUROTOXICITY AT THE N-METHYL-D-ASPARTATE RECEPTOR IN CULTURED NEURONS - PHARMACOLOGICAL CHARACTERIZATION [J].

LYSKO, PG ;

COX, JA ;

VIGANO, MA ;

HENNEBERRY, RC .

BRAIN RESEARCH, 1989, 499 (02) :258-266

[10]

MCGEER EG, 1991, CURR OPIN NEUROL NEU, V4, P548

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