Development of a validated UPLC-qTOE-MS/MS method for determination of bioactive constituent from Glycyrrhiza glabra

被引：12

作者：

Gupta, D. K. ^{[1
]}

Verma, M. K. ^{[2
]}

Anand, R. ^{[2
]}

Khajuria, R. K. ^{[2
]}

机构：

[1] CSIR, Indian Inst Integrat Med, Nat Prod Chem Div, Jammu 180001, India

[2] CSIR, Indian Inst Integrat Med, Analyt Chem Div, Canal Rd, Jammu 180001, India

来源：

JOURNAL OF PHARMACEUTICAL ANALYSIS | 2013年 / 3卷 / 03期

关键词：

Glycyrrhiza glabra; Glycyrrhizin; Glycyrrhetic acid; Accelerated solvent extraction; Ultrasonication; Ultra-performance liquid chromatography quadrupole time of flight mass spectrometer (UPLC-qTOF-MS/MS);

D O I：

10.1016/j.jpha.2013.01.001

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-qTOE-MS/MS) method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile:2% acetic acid in water (75:25, v/v) with a flow rate of 200 mu L/min. The qTOE-MS was operated under multiple reaction monitoring (MRM) mode using the electrospray ionization (ESI) technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction (ASE), extraction under ultrasonic waves (USW) and the classical extraction by percolation (CE) method was done and quantification of these extracts was also carried out by the proposed method. (C) 2013 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. All rights reserved.

引用

页码：205 / 210

页数：6

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