H-3 THYMIDINE UPTAKE BY THE RAT URINARY-BLADDER AFTER PARTIAL OUTFLOW OBSTRUCTION

被引：25

作者：

SAITO, M ^{[1
]}

LONGHURST, PA ^{[1
]}

MURPHY, M ^{[1
]}

MONSON, FC ^{[1
]}

WEIN, AJ ^{[1
]}

LEVIN, RM ^{[1
]}

机构：

[1] UNIV PENN,SCH MED,DIV UROL,PHILADELPHIA,PA 19104

来源：

NEUROUROLOGY AND URODYNAMICS | 1994年 / 13卷 / 01期

关键词：

PARTIAL OBSTRUCTION; RAT BLADDER; H-3 THYMIDINE INCORPORATION; TISSUE PROLIFERATION;

D O I：

10.1002/nau.1930130109

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Partial outflow obstruction induces marked changes in detrusor contractile function and morphology. One common finding in all experimental animal models of outflow obstruction is a significant increase in bladder mass. It is not clear which tissue compartments undergo hypertrophy, hyperplasia, or both. The present study was designed to evaluate the time-related changes in H-3-thymidine uptake and distribution within each tissue compartment induced by partial outflow obstruction using autoradiography. Partial outlet obstruction in rats induced a mild 2-fold increase in mass over a 14 day period. DNA synthesis increased significantly at 1 day following surgery, and remained increased through 7 days. DNA synthesis returned to control levels by 14 days. Distribution studies (using autoradiography) demonstrated a marked increase in the number of labelled urothelium cells at 1 and 3 days after obstruction. Sham surgery also initiated an increase in the number of labelled cells in the urothelium at 1 day. Although both sham and obstructive surgeries induced substantial increases in the labelling of cells within the connective tissue components, the magnitude of the increase in labelled connective tissue cells of the obstructed bladders was greater than that of the sham group. The number of labelled smooth muscle cells of the obstructed bladder increased significantly at 3, 5, and 7 days. However, there were no changes in smooth muscle incorporation of H-3-thymidine by the sham groups. In conclusion, partial outflow obstruction induced time-dependent increases in bladder wall proliferation. The urothelium and connective tissue were the compartments first affected, followed by smooth muscle. (C) 1994 Wiley-Liss, Inc.

引用

页码：63 / 69

页数：7

共 25 条

[11]

Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ, Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder, Prostate (Suppl), 3, pp. 9-26, (1990)

[12]

Malkowicz SB, Wein AJ, Elbadawi A, Van Arsdalen K, Ruggieri MR, Levin RM, Acute biochemical and functional alterations in the partially obstructed rabbit urinary bladder, J Urol, 136, pp. 1324-1329, (1986)

[13]

Mattiasson A, Uvelius B, Changes in contractile properties in hypertrophic rat urinary bladder, J Urol, 128, pp. 1340-1342, (1982)

[14]

Mayo ME, Hinman F, Structure and function of the rabbit bladder altered by chronic obstruction or cystitis, Invest Urol, 14, pp. 6-9, (1976)

[15]

McConnell JD, Lee IL, Cher ML, Expression of nonmuscle caldesmon in obstruction‐induced detrusor hypertrophy, J Urol, 143, (1990)

[16]

McYrick B, Reid L, Hypoxia and incorporation of <sup>3</sup>H‐thymidine by cells of the rat pulmonary arteries and alveolar wall, Am J Pathol, 96, pp. 51-59, (1979)

[17]

Monson FC, Goldschmidt MH, Zderic SA, Ruggieri MR, Levin RM, Wein AJ, Use of a previously undescribed elastic lamina of the serosa to characterize connective tissue hypertrophy of the rabbit bladder wall following partial outflow obstruction, Neurourol Urodyn, 7, pp. 385-396, (1988)

[18]

Monson FC, McKenna BA, Wein AJ, Levin RM, Effect of outlet obstruction on <sup>3</sup>H‐thymidine uptake and metabolism: A radiographic and biochemical study, J Urol, 148, pp. 158-162, (1992)

[19]

Saito M, Longhurst PA, Tammela TL, Wein AJ, Levin RM, Effects of partial outlet obstruction on micturition characteristics, DNA synthesis, and the contractile response to pharmacological agents, J Urol, 150, pp. 1045-1052, (1993)

[20]

See NA, Epstein, Schultz E, Pienkowski TP, Bass P, Hyperplasia of jejunal smooth muscle in the myenterically denervated rat, Cell Tissue Res, 253, pp. 609-614, (1988)

← 1 2 3 →