REDUCTION OF BIOLOGICAL-ACTIVITY OF MURINE RECOMBINANT INTERLEUKIN-1-BETA BY SELECTIVE DEAMIDATION AT ASPARAGINE-149

被引：23

作者：

DAUMY, GO ^{[1
]}

WILDER, CL ^{[1
]}

MERENDA, JM ^{[1
]}

MCCOLL, AS ^{[1
]}

GEOGHEGAN, KF ^{[1
]}

OTTERNESS, IG ^{[1
]}

机构：

[1] PFIZER INC,DIV CENT RES,GROTON,CT 06340

来源：

FEBS LETTERS | 1991年 / 278卷 / 01期

关键词：

INTERLEUKIN-1; CYTOKINE; DEAMIDATION;

D O I：

10.1016/0014-5793(91)80093-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biologically active preparation of murine recombinant interleukin-1-beta (mIL-1-beta) from Escherichia coli cell lysates contained two forms of mIL-1-beta with pI 8.7 and pI 8.1, respectively. Treatment with 0.1 M Tris, pH 8.5, at 37-degrees-C for 35 h converted the pI 8.7 form to the pI 8.1 form by the selective deamidation of an asparagine residue (Asn149) in the mIL-1-beta molecule. Deamidated mIL-1-beta had 3- to 5-fold lower co-mitogenic activity and receptor affinity than the unmodified form.

引用

页码：98 / 102

页数：5

共 27 条

[21] THE PHARMACOLOGIC REGULATION OF INTERLEUKIN-1 PRODUCTION - THE ROLE OF PROSTAGLANDINS [J].