IDENTIFICATION OF A SURFACE PROTEIN (P100) ASSOCIATED WITH 2 GLYCOSYL-PHOSPHATIDYLINOSITOL-LINKED MOLECULES (THY-1 AND THB) BY NATURAL ANTILYMPHOCYTE AUTOANTIBODIES

被引:11
作者
LEHUEN, A
MONTEIRO, RC
KEARNEY, JF
机构
[1] UNIV ALABAMA,DEPT MICROBIOL,DIV DEV & CLIN IMMUNOL,BIRMINGHAM,AL 35294
[2] UNIV ALABAMA,CTR COMPREHENS CANC,BIRMINGHAM,AL 35294
关键词
D O I
10.1002/eji.1830220929
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Our previous study of natural autoantibodies showed that anti-lymphocyte antibodies are frequently produced by perinatal B cells from normal strains of mice. One-third of these monoclonal antibodies (mAb) recognized similar epitopes on the surface of thymocytes. In the present report, we have characterized the molecule recognized by three of these mAb (D10, G7, 22). These mAb identified a 100-kDa protein (p100) on the surface of thymocytes. This protein resolved into 70-kDa polypeptide chains under reducing conditions. Inhibition experiments as well as antibody immunoprecipitations in the presence of mild detergents revealed non-covalent association of the p 100 with Thy-1 and ThB. A similar multimolecular complex was identified following chemical cross-linking of thymocyte surface proteins. Analysis of several Thy-1-defective mutant cells lines, and thymocytes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) showed that the expression of p100 was strongly influenced by Thy-1 molecule. The p 100 was resistant to PI-PLC treatment and was not released into the supernatant as was the case for Thy-1 and ThB molecules. These data lead us to propose that the p100 is a transmembrane protein, the expression of which in the plasma membrane is dependent on the association or presence of Thy-1 molecule.
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页码:2373 / 2380
页数:8
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