RECEPTORS FOR PEPTIDE YY AND NEUROPEPTIDE-Y ON GUINEA-PIG PANCREATIC ACINI

被引：14

作者：

HUANG, SC ^{[1
]}

TSAI, MF ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,DEPT PHYSIOL,TAIPEI 100,TAIWAN

来源：

PEPTIDES | 1994年 / 15卷 / 03期

关键词：

PEPTIDE YY; NEUROPEPTIDE Y; VASOACTIVE INTESTINAL POLYPEPTIDE; FORSKOLIN; CHOLECYSTOKININ; AMYLASE RELEASE; CYCLIC AMP;

D O I：

10.1016/0196-9781(94)90196-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the effects of peptide YY (PYY), neuropeptide Y (NPY), and their analogues on dispersed pancreatic acini. Binding of [I-125]PYY to acini was saturable, reversible, and specific, and PYY binding was best fit with a two-site model. The relative potencies for inhibiting [I-125]PYY binding were PYY greater than or equal to NPY > NPY(13-36). There was no inhibition of binding with [Leu(31),Pro(34)]NPY, PYX-2, or pancreatic polypeptide. Both PYY and NPY (0.1 mu M) inhibited amylase release stimulated by vasoactive intestinal polypeptide (VIP) (0.3 nM) and forskolin (1 mu M) by about 30%, but not that stimulated by cholecystokinin-8 or bombesin. The relative potencies for inhibiting VIP-stimulated amylase release were PYY greater than or equal to NPY > NPY(13-36), the same as those for inhibiting VIP-stimulated cAMP increase in acini. No inhibition was detected with [Leu(31),Pro(34)]Npy. This work demonstrates Y-2 receptors on guinea pig pancreatic acini mediating inhibitory actions of PYY and NPY on pancreatic enzyme secretion.

引用

页码：405 / 410

页数：6

共 35 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] A NEW AND RAPID METHOD FOR CLINICAL DETERMINATION OF ALPHA-AMYLASE ACTIVITIES IN HUMAN SERUM AND URINE . OPTIMAL CONDITIONS [J].