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RAB AND RHO GTPASES ARE INVOLVED IN SPECIFIC RESPONSE OF PERIODONTAL-LIGAMENT FIBROBLASTS TO MECHANICAL STRETCHING

被引：40
作者：
BASDRA, EK
PAPAVASSILIOU, AG
HUBER, LA
机构：
[1] UNIV HEIDELBERG,POLIKLIN KIEFERORTHOPAD,D-69120 HEIDELBERG,GERMANY
[2] EUROPEAN MOLEC BIOL LAB,DIFFERENTIAT PROGRAMME,D-69012 HEIDELBERG,GERMANY
[3] UNIV GENEVA,DEPT BIOCHEM,CH-1211 GENEVA 4,SWITZERLAND
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1268卷 / 02期
关键词：
PERIODONTAL LIGAMENT FIBROBLAST; MECHANICAL STRETCHING; MECHANOTRANSDUCTION; GTPASE; SMALL; RHO; RAB;
D O I：
10.1016/0167-4889(95)00090-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate the contribution of ras-related signalling molecules to the mechanotransduction process, stretch-sensing human periodontal ligament (PDL) fibroblasts were isolated and cultured in dishes with a flexible bottom. The cells were stimulated by stretching the bottom of the dishes and membrane fractions were prepared and analysed at the level of mapping small GTP-binding proteins by high resolution two-dimensional gel electrophoresis followed by renaturing transfer and an [alpha-P-32]GTP-overlay procedure. This analysis revealed that mechanically-stretched PDL fibroblasts exhibit complete down-regulation of rhoA and induction of rab6, rab17 and a putative member of the rab3 subfamily in a cell type-specific manner.
引用
收藏
页码：209 / 213
页数：5
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