SPECIES-SPECIFIC 18S RIBOSOMAL-RNA GENE AMPLIFICATION FOR THE DETECTION OF PLASMODIUM-FALCIPARUM AND PLASMODIUM-VIVAX MALARIA PARASITES

被引:40
作者
DAS, A
HOLLOWAY, B
COLLINS, WE
SHAMA, VP
GHOSH, SK
SINHA, S
HASNAIN, SE
TALWAR, GP
LAL, AA
机构
[1] ALL INDIA INST MED SCI,DEPT BIOCHEM,NEW DELHI 110029,INDIA
[2] NATL INST IMMUNOL,NEW DELHI 110067,INDIA
[3] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV PARASIT DIS,MALARIA BRANCH,ATLANTA,GA 30341
[4] CDC,NCID,SCI RESOURCE BRANCH,BIOTECHNOL CORE FACIL,ATLANTA,GA
[5] INDIAN COUNCIL MED RES,MALARIA RES CTR,NEW DELHI,INDIA
关键词
DIAGNOSIS; MALARIA; POLYMERASE CHAIN REACTION; RIBOSOMAL RNA;
D O I
10.1006/mcpr.1995.0025
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Based on the sequence diversity of the Plasmodium 18S ribosomal RNA (rRNA), we designed oligonucleotide primers for polymerase chain reaction (PCR) to yield different size fragments for P. falciparum and P. vivax. The primers for the PCR procedure were chosen such that the 5' primer was Plasmodium-conserved while the 3' primers were species-specific. Using primer cocktails and cloned plasmid DNAs containing the 18S rRNA genes or parasite genomic DNA as targets, we show that the PCR procedure yields 1.4-kb and 0.5-kb DNA fragments for P. falciparum and P. vivax, respectively. Limited field testing of this procedure demonstrated the utility of a ribosomal gene based species-specific malaria diagnosis.
引用
收藏
页码:161 / 165
页数:5
相关论文
共 13 条
[1]  
The biology of malaria parasites, WHO Technical Report Series, 743, pp. 157-158, (1987)
[2]  
Spencer H.C., Strickland C.T., Malaria, Hunters Tropical Medicine, pp. 516-552, (1984)
[3]  
Kawamoto F., Rapid diagnosis of malaria by fluorescence microscopy with light microscope and interference filter, Lancet, 336, pp. 200-202, (1991)
[4]  
Lanar D.E., McLaughlin G.L., Wirth D.F., Barker R.D., Zolg J.W., Chulay J.D., Comparison of thick films, in vitro culture and DNA hybridization probes for detecting Plasmodium falciparum malaria, American Journal of Tropical Medicine and Hygiene, 40, pp. 3-6, (1989)
[5]  
McCutchan T.F., The ribosomal genes of Plasmodium, International Review of Cytology, 99, pp. 295-309, (1986)
[6]  
McCutchan T.F., Dela Cruz V.F., Lai A.A., Gunderson D.H., Elwood H.J., Sogin M.L., The primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum, Molecular and Biochemical Parasitology, 28, pp. 63-68, (1987)
[7]  
Waters A.P., McCutchan T.F., Partial sequence of the asexually expressed small subunit ribosomal RNA genes from Pvivax, Nucleic Acid Research, 17, pp. 2135-2136, (1989)
[8]  
Lai A.A., Changkasiri S., Hollingdale M.R.R., McCutchan T.F., Ribosomal RNA based diagnosis of Plasmodium falciparum malaria, Molecular and Biochemical Parasitology, 36, pp. 67-72, (1989)
[9]  
Waters A.P., McCutchan T.F., Rapid and sensitive diagnosis of malaria based on ribosomal RNA, Lancet, 5, pp. 1343-1346, (1989)
[10]  
Macallum F.S., Maden B.E.H., Human 18S ribosomal RNA sequence inferred from DNA sequence, Biochemical Journal, 232, pp. 725-733, (1985)