SPECIES-SPECIFIC 18S RIBOSOMAL-RNA GENE AMPLIFICATION FOR THE DETECTION OF PLASMODIUM-FALCIPARUM AND PLASMODIUM-VIVAX MALARIA PARASITES

被引：40

作者：

DAS, A

HOLLOWAY, B

COLLINS, WE

SHAMA, VP

GHOSH, SK

SINHA, S

HASNAIN, SE

TALWAR, GP

LAL, AA

机构：

[1] ALL INDIA INST MED SCI,DEPT BIOCHEM,NEW DELHI 110029,INDIA

[2] NATL INST IMMUNOL,NEW DELHI 110067,INDIA

[3] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV PARASIT DIS,MALARIA BRANCH,ATLANTA,GA 30341

[4] CDC,NCID,SCI RESOURCE BRANCH,BIOTECHNOL CORE FACIL,ATLANTA,GA

[5] INDIAN COUNCIL MED RES,MALARIA RES CTR,NEW DELHI,INDIA

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 03期

关键词：

DIAGNOSIS; MALARIA; POLYMERASE CHAIN REACTION; RIBOSOMAL RNA;

D O I：

10.1006/mcpr.1995.0025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the sequence diversity of the Plasmodium 18S ribosomal RNA (rRNA), we designed oligonucleotide primers for polymerase chain reaction (PCR) to yield different size fragments for P. falciparum and P. vivax. The primers for the PCR procedure were chosen such that the 5' primer was Plasmodium-conserved while the 3' primers were species-specific. Using primer cocktails and cloned plasmid DNAs containing the 18S rRNA genes or parasite genomic DNA as targets, we show that the PCR procedure yields 1.4-kb and 0.5-kb DNA fragments for P. falciparum and P. vivax, respectively. Limited field testing of this procedure demonstrated the utility of a ribosomal gene based species-specific malaria diagnosis.

引用

页码：161 / 165

页数：5

共 13 条

[11]

Sambrook J., Fritsch E.F., Maniatis T.F., Molecular Cloning: A Laboratory Manual, (1989)

[12]

Warhurst D.C., Awad El-Kariem F.M., Miles M.A., Simplified preparation of malarial blood samples for polymerase chain reaction, Lancet, 337, pp. 303-304, (1991)

[13]

Snounou G., Viriyakosol S., Jarra W., Thaithong S., Brown K., Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections, Molecular and Biochemical Parasitology, 58, pp. 283-292, (1993)

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