EFFECT OF ATP DEPLETION AND PHENANTHROLINE ON THE SPERMIDINE-MEDIATED DECAY OF ORNITHINE DECARBOXYLASE IN ERYTHROLEUKEMIA-CELLS

被引：4

作者：

FLAMIGNI, F ^{[1
]}

MARMIROLI, S ^{[1
]}

GUARNIERI, C ^{[1
]}

CALDARERA, CM ^{[1
]}

机构：

[1] IST RIZZOLI,CNR,IST CITOMORFOL,I-40136 BOLOGNA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90766-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Addition of spermidine to Friend erythroleukemia cells caused a rapid decay of ornithine decarboxylase (ODC) activity and the accumulation of a ODC-antizyme complex. The induction of antizyme only partially accounted for the decrease of ODC activity by a direct inhibition of the enzyme. However, the antizyme induction was accompanied by a marked reduction of the half-life of ODC. Shift of the cells to an ATP-depleting medium prevented the spermidine-elicited decay of ODC activity as well as the accumulation of ODC-antizyme complex. However, ODC appeared to be stabilized even when ATP depletion was performed 40 min after spermidine addition, in the presence of high levels of antizyme. Similar results were obtained by treating the cells with phenanthroline, a heavy metal chelator and protease inhibitor. These findings indicate that ATP and some metalloprotease(s) may be involved in the degradation pathway of ODC, even in the presence of high levels of polyamines. © 1990.

引用

页码：939 / 944

页数：6

共 27 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

CANELLAKIS ES, 1988, PHYSL POLYAMINES, P315

[3] UBIQUITIN DEPENDENCE OF SELECTIVE PROTEIN-DEGRADATION DEMONSTRATED IN THE MAMMALIAN-CELL CYCLE MUTANT TS85 [J].