IDENTIFICATION AND CHARACTERIZATION OF REGULATORY ELEMENTS IN THE PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE PCK1 OF SACCHAROMYCES-CEREVISIAE

被引：42

作者：

PROFT, M ^{[1
]}

GRZESITZA, D ^{[1
]}

ENTIAN, KD ^{[1
]}

机构：

[1] UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,INST MIKROBIOL,D-60439 FRANKFURT,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 246卷 / 03期

关键词：

SACCHAROMYCES CEREVISIAE; PHOSPHOENOLPYRUVATE CARBOXYKINASE; GLUCOSE REPRESSION; GLUCONEOGENESIS;

D O I：

10.1007/BF00288610

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoenolpyruvate carboxykinase is a key enzyme in gluconeogenesis. The expression of the PCK1 gene in Saccharomyces cerevisiae is strictly regulated and dependent on the carbon source provided. Two upstream activation sites (UAS1(PCK1) and UAS2(PCK1)) and one upstream repression site (URS(PCK1)) were localized by detailed deletion analysis. The efficacy of these three promoter elements when separated from each other was confirmed by investigations using heterologous promoter test plasmids. Activation mediated by UAS1(PCK1) or UAS2(PCK1) did not occur in the presence of glucose, indicating that these elements are essential for glucose derepression. The repressing effect caused by URS(PCK1) was much stronger in glucose-grown cells than in ethanol-grown cells.

引用

页码：367 / 373

页数：7