DETECTION OF VARIOUS VARIANT VEROTOXIN GENES IN ESCHERICHIA-COLI BY POLYMERASE CHAIN-REACTION

被引：110

作者：

LIN, Z ^{[1
]}

KURAZONO, H ^{[1
]}

YAMASAKI, S ^{[1
]}

TAKEDA, Y ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT MICROBIOL,SAKYO KU,KYOTO,KYOTO 60601,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1993年 / 37卷 / 07期

关键词：

VEROTOXIN GENE; POLYMERASE CHAIN REACTION (PCR);

D O I：

10.1111/j.1348-0421.1993.tb01675.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We constructed common primers for the polymerase chain reaction to detect the genes for various Verotoxins reported, that is, VT1 (or SLT-I), VT2 (or SLT-II), VT2vha, VT2vhb, SLT-IIv (or VT2vpl, VTe) and SLT-IIva (or VT2vp2). A total of 80 Verocytotoxin-producing Escherichia coli strains isolated from humans, domestic animals and meats gave a positive result by PCR with the designed common primers. Digestion by restriction endonucleases BglII and EcoT141 of the amplicon of the VT2vp2 gene gave specific bands of the expected sizes, but not of the amplicons of other VT genes, suggesting a possible method for identification of the VT2vp2 gene. Application of the PCR with the designed primers in diagnostic and epidemiological studies on VTEC infection is

引用

页码：543 / 548

页数：6

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