Functional elimination of calmodulin within the nucleus by targeted expression of an inhibitor peptide

被引：62

作者：

Wang, JH ^{[1
]}

Campos, B ^{[1
]}

Jamieson, GA ^{[1
]}

Kaetzel, MA ^{[1
]}

Dedman, JR ^{[1
]}

机构：

[1] UNIV CINCINNATI,DEPT CELLULAR & MOLEC PHYSIOL,CINCINNATI,OH 45267

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 51期

关键词：

D O I：

10.1074/jbc.270.51.30245

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic manipulation has proven valuable in identifying the role of specific genes in cellular function. Genomic disruption of genes that are expressed during embryonic development or in multiple tissue types, however, complicates phenotypic analysis. We demonstrate that targeted expression of an inhibitor peptide derived from myosin light chain kinase can neutralize the function of calmodulin. We have shown that elimination of the nuclear function of Ca2+-calmodulin causes disruption of the nuclear structure. Targeted expression of this calmodulin inhibitor gene in the lung epithelium of transgenic mice leads to cellular death and dysfunctional lung development. This approach is a strategy to modify the activity of a targeted protein within a specific organelle in order to evaluate its role in cellular and tissue function.

引用

页码：30245 / 30248

页数：4