CLASSICAL SWINE FEVER VIRUS-SPECIFIC CYTOTOXIC T-LYMPHOCYTES AND IDENTIFICATION OF A T-CELL EPITOPE

被引：105

作者：

PAULY, T ^{[1
]}

ELBERS, K ^{[1
]}

KONIG, M ^{[1
]}

LENGSFELD, T ^{[1
]}

SAALMULLER, A ^{[1
]}

THIEL, HJ ^{[1
]}

机构：

[1] FED RES CTR VIRUS DIS ANIM, W-7400 TUBINGEN, GERMANY

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-12-3039

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Classical swine fever virus (CSFV)-specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4(-)CD6(+)CD8(+) MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.

引用

页码：3039 / 3049

页数：11

共 44 条

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