CLONING, SEQUENCING AND FUNCTIONAL EXPRESSION OF AN ACETYLCHOLINESTERASE GENE FROM THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI

被引：52

作者：

ANTHONY, N

ROCHELEAU, T

MOCELIN, G

LEE, HJ

FFRENCHCONSTANT, R

机构：

[1] UNIV WISCONSIN, DEPT NEUROSCI, MADISON, WI 53706 USA

[2] UNIV WISCONSIN, DEPT ENTOMOL, MADISON, WI 53706 USA

来源：

FEBS LETTERS | 1995年 / 368卷 / 03期

关键词：

ACETYLCHOLINESTERASE; YELLOW FEVER MOSQUITO; AEDES AEGYPTI; BACULOVIRUS EXPRESSION; INSECTICIDE RESISTANCE;

D O I：

10.1016/0014-5793(95)00711-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A degenerate PCR strategy was used to isolate a fragment of the acetylcholinesterase gene (Ace) homolog from Aedes aegypti and screen for a cDNA clone containing the complete open reading frame of the gene. The predicted amino acid sequence of the Aedes gene shares 64% identity with Ace from Dr osophila and 87% identity with the acetylcholinesterase gene from another mosquito species Anopheles stephensi. High levels of expression of the Aedes gene were achieved by infection of Sf21 cells with a recombinant baculovirus containing the Aedes Ace cDNA. The catalytic properties and sensitivity of the recombinant enzyme to insecticide inhibition are described and discussed in relation to the role of insensitive AChE in conferring resistance to organophosphorus and carbamate insecticides.

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页码：461 / 465

页数：5

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